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Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
UseTags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
huCaV2.2 (pSAD442-1)
Plasmid#62574PurposeHuman CaV2.2 expression in mammalian cellsDepositorInsertCACNA1B (CACNA1B Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRmine-mScarlet-WPRE
Plasmid#130994PurposeAAV vector to drive the expression of ChRmine-mScarlet under the control of human synapsin promoterDepositorInsertChRmine
UseAAVTagsmScarletExpressionMutationPromoterhSynAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
DsRed2-ER-5
Plasmid#55836PurposeLocalization: Endoplasmic Reticulum, Excitation: 558, Emission: 583DepositorInsertER (CALR Human)
UseTagsDsRed2ExpressionMammalianMutationER Signal PeptidePromoterCMVAvailable sinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pY016 (pcDNA3.1-hLbCpf1)
Plasmid#69988PurposeExpresses humanized LbCpf1DepositorInserthLbCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-sgRNA-RFP
Plasmid#166005PurposeBacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pRDA_886
Plasmid#201162PurposeLentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLSDepositorInsertPuromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
[pSK600] pRK5 HA-Nprl2(R78A)
Plasmid#136146Purposemammalian expression of Nprl2DepositorInsertNprl2 (NPRL2 Human)
UseTagsHAExpressionMammalianMutationR78APromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-inactive M3nls
Plasmid#157854PurposeTargeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutantDepositorInsertdCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
UseTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133A; METTL3 D395APromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDule-para-aminoPhe
Plasmid#85502PurposePlasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertp-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsNoneExpressionBacterialMutationY32T, E107T, D158P, I159L, L162APromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ADAM10-HA
Plasmid#65106PurposeExpression of HA tagged ADAM10 in mammalian cellsDepositorInsertADAM10 (ADAM10 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC7A5
Plasmid#132197PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC7A5 (SLC7A5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-sCAG-mBaoJin-oPRE
Plasmid#218684PurposeAAV vector plasmid expressing mBaoJin under a short variant of the CAG (sCAG) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.DepositorInsertmBaoJin
UseAAVTagsExpressionMammalianMutationPromoterShort variant of the CAG promoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_ATP1.0
Plasmid#167577PurposeExpress the ATP sensor GRAB_ATP1.0 in neuronsDepositorInsertATP sensor GRAB_ATP1.0 (P2RY1 Human)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLGB29
Plasmid#126619PurposeSuicide vector for allelic replacement in Bacteroides, inulin selection and aTC-inducible ss-Bfe1 counterselectionDepositorTypeEmpty backboneUseAllellic exchange in bacteriaTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 EGFP-Tau
Plasmid#108868PurposeExpresses EGFP linked to human TauDepositorInsertTau (MAPT Human)
UseLentiviralTagsEGFPExpressionMammalianMutationhuman four-repeat tau lacking the N-terminal sequ…PromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IDH1
Plasmid#116751PurposeLentiviral expression of IDH1DepositorInsertIDH1 (IDH1 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_C1
Plasmid#85042PurposeMammalian expression of mScarletDepositorHas ServiceCloning Grade DNAInsertmScarlet
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_NoFlag_hROSA26_Left
Plasmid#191442PurposeExpresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locusDepositorInserthROSA26 sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-As-crRNA
Plasmid#78956PurposeCloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
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LRG2.1
Plasmid#108098PurposeLentiviral expression plasmid of sgRNA with GFPDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for sgRNA expression and EFS promoter…Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRAF_p.V600E
Plasmid#82186PurposeGateway Donor vector containing BRAF , part of the Target Accelerator Plasmid Collection.DepositorInsertBRAF (BRAF Human)
UseGateway entry vectorTagsExpressionMutationV600EPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-inactive M3M14nes
Plasmid#157855PurposeTargeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutantDepositorInsertUseTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133A; METTL3 D395APromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI NL4-3 Nef-HA-WT
Plasmid#24162DepositorInsertNef (nef HIV)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IDH1-R132C
Plasmid#116410PurposeLentiviral expression of IDH1 R132CDepositorInsertIDH1 (IDH1 Human)
UseLentiviralTagsExpressionMutationR132CPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SEP-Glur2 (MCA10)
Plasmid#64941Purposemammalian expression of rat GluR2DepositorInsertGluR2 (Gria2 Rat)
UseTagsSEP (superecliptic pHluorin)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
plentiCMV-sct-pp65-b2m-HLA-A0201
Plasmid#196511PurposeExpress single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)DepositorInsertsingle chain of a pp65 peptide, b2m and HLA A0201
UseLentiviralTagsNAExpressionMammalianMutationY84CPromoterCMVAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.eGFP.WPRE.hGH
Plasmid#105549PurposeAAV expression of EGFP from GFAP promoterDepositorHas ServiceAAV5InserteGFP
UseAAVTagsExpressionMammalianMutationPromoterGFAPAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only