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Plasmid#183987PurposeExpresses fluorescent HaloTag7 fusion protein in mammalian cells with localization in the ER.DepositorInsertCalnexin (CANX Human)
UseTagsHaloTag, mGoldExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato-NLS. PGK-puro
Plasmid#136261PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomato-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFPc2 mAbp1
Plasmid#26719DepositorInsertActin-binding Protein-1 (Dbnl Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
p5E-0.349cldn15la
Plasmid#125026PurposeFive prime entry vector containing 349bp cldn15la promoter from zebrafish. Drives expression in intestinal epithelial cells.DepositorInsertClaudin 15-like a (cldn15la Zebrafish)
UseTagsExpressionMutationPromoterAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL3 CTF-FLAG
Plasmid#217708PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL3 (ADGRL3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-841PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRB1 CTF-FLAG
Plasmid#217681PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRB1 (ADGRB1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-926PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG5 CTF-FLAG
Plasmid#217703PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG5 (ADGRG5 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-226PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF4 CTF-FLAG
Plasmid#217697PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF4 (ADGRF4 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-384PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-BICD1
Plasmid#49487Purposeexpresses EGFP-BICD1 in mammalian cellsDepositorInsertBICD1 (BICD1 Human)
UseTagsEGFPExpressionMammalianMutation** see notesPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJG85
Plasmid#89281PurposeEntry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sitesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Nkx6.2
Plasmid#64846PurposeA lentiviral vector for the expression of mouse Nkx6.2 under doxycyline controlDepositorInsertNkx6.2 (Nkx6-2 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_ST18
Plasmid#64844PurposeA lentiviral vector for the expression of mouse ST18 under doxycyline controlDepositorInsertST18 (St18 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI-globin_del5UTR_WT-xrRNA-4H
Plasmid#108366PurposeExpresses wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertWild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAcCas12nHS_RV6_PT1_empty
Plasmid#203810PurposePlasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.DepositorInsertAcCas12nHS_RV6_PT1
UseCRISPRTagsExpressionMutationPromoterCMVAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcCas12nHS_ABE_D3_empty
Plasmid#203809PurposePlasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).DepositorInsertABE_D3
UseCRISPRTagsExpressionMutationPromoterCMVAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBstSK+IRES-V5-IPpoI
Plasmid#206321PurposeA plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoIDepositorInsertIRES element-V5 epitope tag- IPpoI open reading frame
UseMrna productionTagsV5-IPpoI fusion proteinExpressionMutationPromoterAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4H
Plasmid#108375PurposeFRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertWild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
GOMO-GFP-LUC
Plasmid#60976PurposeRapid selection of recombinant marker-free bioluminescent rodent malaria parasitesDepositorInsertsGFP-Luciferase fusion
mCherry
hDHFR-yFCU
UseTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMTS-hFluc-HA-GFP11
Plasmid#177726PurposeFirefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFluc
UseTagsGFP11, HA, and Mitochondrial Targeting SequenceExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
FoxO1 KR
Plasmid#66166PurposeExpresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by arginine residues.DepositorInsertFoxO1 KR (Foxo1 Mouse)
UseRetroviralTagsExpressionMammalianMutationK242R, K245R and K262RPromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only