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pAc5.1B-EGFP-DmCup-del580-600_O

Plasmid

#147101

Purpose

Insect Expression of DmCup-del580-600

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCup-del580-600 (cup Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCup-L379AL383A_O

Plasmid

#147102

Purpose

Insect Expression of DmCup-L379AL383A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCup-L379AL383A (cup Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 F355R

Plasmid

#195209

Purpose

Mammalian expression vector for GFP tagged NHERF1. Ezrin interaction-deficient mutant

Depositor

David Bryant, Anthony Bretscher

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

F355R

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 PDZ1*

Plasmid

#195210

Purpose

Mammalian expression vector for GFP tagged NHERF1. PDZ1 GYGF>GYAA motif mutant

Depositor

David Bryant, Anthony Bretscher

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

PDZ1 GYGF>GYAA

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 PDZ2*

Plasmid

#195212

Purpose

Mammalian expression vector for GFP tagged NHERF1. PDZ2 GYGF>GYAA motif mutant

Depositor

David Bryant, Anthony Bretscher

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

PDZ2 GYGF>GYAA

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSilencer2.1-U6-T1-U6-bc339

Plasmid

#190849

Purpose

For mammalian expression of T1 and bc339, the RNA aptamers of ncOGT and β-catenin, respectively. Expression of each aptamer is driven by a U6 promoter.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Inserts

T1 (an RNA aptamer of O-GlcNAc Transferase)
bc339 (an RNA aptamer of β-catenin)

Use

Affinity Reagent/ Antibody and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pXD70LacZ2-Pdadh-LacZ

Plasmid

#191618

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70LacZ4-PdegV-LacZ

Plasmid

#191620

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), PdegV promoter-lacZ, E. lenta constitutive promoter

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70LacZ8.1-Pcadh-LacZ

Plasmid

#191624

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70LacZ9-Phcdh-LacZ

Plasmid

#191625

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZ

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadRmt1)-DadR(ΔDBD)

Plasmid

#191637

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔDBD) complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Use

Tags

Expression

Bacterial

Mutation

ΔDBD

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadRmt2)-DadR(ΔTM)

Plasmid

#191638

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔTM) complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Use

Tags

Expression

Bacterial

Mutation

ΔTM

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(HcdR)-HcdR

Plasmid

#191648

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), hcdR complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

HcdR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(CadR)-CadR

Plasmid

#191649

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), cadR complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CadR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF

Plasmid

#177267

Purpose

CRISPR vector

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCK411.RR1

Plasmid

#192644

Purpose

Expresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

BBa_J23119-RR1

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

BBa_J23119

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pRB121

Plasmid

#181945

Purpose

aTc-inducible expression of dimerized mNeonGreen (mNG-mNG)

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

mNeonGreen-mNeonGreen; tetR (AX04_RS23485 Synthetic)

Use

Tags

Expression

Bacterial

Mutation

See Depositor Comments Below

Promoter

mNG-mNG-PLtetO-1; tetR-J23106

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-58-DmeIF4Ga_687-1666_T

Plasmid

#147580

Purpose

Insect Expression of Dm4E-T_1-58-DmeIF4Ga_687-1666

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-58-DmeIF4Ga_687-1666 (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2028

Plasmid

#179771

Purpose

Pnlf-1::NLF::2XGFP unc-54 3'UTR C.elegans neural expression of NLF::2XGFP

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1::2XGFP (nlf-1 Nematode)

Use

Tags

2XGFP

Expression

Worm

Mutation

Promoter

nlf-1

Available since

March 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2198

Plasmid

#179773

Purpose

Prgef-1 NLF-1::GFP unc-54 3' UTR C.elegans pan-neural expression of NLF-1::GFP

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1::GFP (nlf-1 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Prgef-1

Available since

March 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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