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Plasmid#74342PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx3. Not for gene expression.DepositorInsertdistal-less homeobox 3 (Dlx3 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPAct2noUTR (GB0192)
Plasmid#68177PurposeProvides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid partDepositorInsertAtAct2 promoter (no UTR)
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPAtUbq10 (GB0223)
Plasmid#68174PurposeProvides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid partDepositorInsertAtUbq10 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF
Plasmid#72949PurposeEntry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction siteDepositorInsertLevel 0 - ORFs
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Meis2
Plasmid#74349PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Meis2. Not for gene expression.DepositorInsertMeis homeobox 2 (Meis2 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHcPro (GB0067)
Plasmid#68196PurposeProvides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid partDepositorInsertTEV HcPro coding region
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pISH-Cux1
Plasmid#74341PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Cux1. Not for gene expression.DepositorInsertcut-like homeobox 1 (Cux1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Adnp
Plasmid#74340PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Adnp. Not for gene expression.DepositorInsertactivity-dependent neuroprotective protein (Adnp Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTAtUbq3 (GB0219)
Plasmid#68192PurposeProvides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid partDepositorInsertAtUbq3 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Gucy1b3
Plasmid#105458Purposeto generate a riboprobe for in situ hybridazationDepositorInsertGucy1b3 (Gucy1b1 Mouse)
UseTo ganerate riboprobeTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pISH-Meis1
Plasmid#74348PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Meis1. Not for gene expression.DepositorInsertMeis homeobox 1 (Meis1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFL-TK-AID3'UTR-MUT
Plasmid#19987DepositorInsertAID 3' UTR (Aicda Mouse)
UseTagsExpressionMammalianMutationMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pCINTBeta1
Plasmid#14067DepositorInsertChicken Beta 1 Integrin (ITGB1 Chicken)
UseTagsExpressionMammalianMutationPstI-BglI fragment was blunt end cloned and EcoRI…PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDualEX-SMN2
Plasmid#214431PurposeDual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7DepositorInsertSMN2 EXON 7 (SMN2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
LacI pm
Plasmid#218097PurposePhagemid that expresses LacI but no phage proteins, used as a controlDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMB1-C-TelR
Plasmid#204981PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB1-D-TelR
Plasmid#204982PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB1-E-TelR
Plasmid#204984PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only