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Plasmid#165049PurposeIntegrative expression of TRX1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>TRX1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID8Z105
Plasmid#165057PurposeIntegrative expression of nucleus-targeting yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID9L105
Plasmid#165055PurposeIntegrative expression of nucleus-targeting yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID9L
Plasmid#165052PurposeIntegrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiaeDepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID5Z
Plasmid#165047PurposeIntegrative expression of AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…PromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Bth
Plasmid#129686Purpose(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoterDepositorInsertEmpty Backbone
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWDY1226
Plasmid#138593PurposeExpresses NatL2-R156A in E.coliDepositorInsertphenylacetate-coenzyme A ligase
UseTags6-HisExpressionBacterialMutationchanged Arginine 156 to AlaninePromoterT7Available sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWDY1222
Plasmid#138592PurposeExpresses NatL2-K418A in E.coliDepositorInsertphenylacetate-coenzyme A ligase
UseTags6-HisExpressionBacterialMutationchanged Lysine 418 to AlaninePromoterT7Available sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Bh
Plasmid#136378PurposeGateway entry clone for BhCas12b sgRNA expression under ZmUbi promoter with ribozyme processingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Ace1_Q89D_D100N_E206V-HaloTag
Plasmid#129255PurposeExpress Ace1_Q89D_D100N_E206V-HaloTag in mammalian cellsDepositorInsertAce1_Q89D_D100N_E206V-HaloTag
UseTagsExpressionMammalianMutationQ89D, D100N, E206VPromoterCAGAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Mac_Q139D_D150N_E258V-HaloTag-ST
Plasmid#129264PurposeExpress soma-localized Mac_Q139D_D150N_E258V-HaloTag in mammalian cellsDepositorInsertMac_Q139D_D150N_E258V-HaloTag-ST
UseTagsExpressionMammalianMutationQ139D,D150N,E258VPromoterCAGAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag-ST
Plasmid#129263PurposeExpress soma-localized QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cellsDepositorInsertQuaAr3_Q95D_H106N_E214V-HaloTag-ST
UseTagsExpressionMammalianMutationQ95D, H106N, E214VPromoterCAGAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Ace1_Q89D_D100N_E206V-HaloTag-ST
Plasmid#129260PurposeExpress soma-localized Ace1_Q89D_D100N_E206V-HaloTag in mammalian cellsDepositorInsertAce1_Q89D_D100N_E206V-HaloTag-ST
UseTagsExpressionMammalianMutationQ89D, D100N, E206VPromoterCAGAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Mac_Q139D_D150N_E258V-HaloTag
Plasmid#129259PurposeExpress Mac_Q139D_D150N_E258V-HaloTag in mammalian cellsDepositorInsertMac_Q139D_D150N_E258V-HaloTag
UseTagsExpressionMammalianMutationQ139D,D150N,E258VPromoterCAGAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag
Plasmid#129258PurposeExpress QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cellsDepositorInsertQuaAr3_Q95D_H106N_E214V-HaloTag
UseTagsExpressionMammalianMutationQ95D, H106N, E214VPromoterCAGAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/68
Plasmid#113921PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68DepositorInsertMBP-NanoLucCP-65/68
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/66
Plasmid#113920PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66DepositorInsertMBP-NanoLucCP-65/66
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-LUCIDRS-MTX
Plasmid#113925PurposePlasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic biosensor for methotrexateDepositorInsertLUCID_RS-MTX
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7T3D-PacI-Tmeff2
Plasmid#99728Purposeto make in situ RNA probes for Tmeff2DepositorInsertTmeff2 (Tmeff2 Mouse)
UseIn situ probeTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
POT5-TDH3p-ycrtYB-ADH1t
Plasmid#65358PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertTDH3p-ycrtYB-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only