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Plasmid#69308PurposeGateway entry vector with hSpCas9D10ADepositorInserthSpCas9D10A (Human codon optimized)
UseCRISPRTags3XFLAGExpressionMutationD10A mutation; NLS fusionPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_H303A
Plasmid#67730PurposeExpresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
UseTags6x-His tagExpressionBacterialMutationChanged histidine 303 to alaninePromoterT7Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_D230A
Plasmid#67725PurposeExpresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
UseTags6x-His tagExpressionBacterialMutationChanged aspartate 230 to alaninePromoterT7Available sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_N191A
Plasmid#67724PurposeExpresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
UseTags6x-His tagExpressionBacterialMutationChanged asparagine 191 to alaninePromoterT7Available sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_K295A
Plasmid#67726PurposeExpresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
UseTags6x-His tagExpressionBacterialMutationChanged lysine 295 to alaninePromoterT7Available sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSB4C5_J23101-CocE
Plasmid#128129PurposeExpressing CocE enzyme (cocaine to benzoate) gene under control of the constitutive promoter J23101, and RBS B0032DepositorInsertCocE
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrc-clbS_Bifido
Plasmid#223359PurposeEncoding ClbS from Bifidobacterium longum subsp. infantisDepositorInsertEncoding ClbS from Bifidobacterium longum subsp. infantis (Codon optimized for E coli)
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc-clbS_Samsonia
Plasmid#223357PurposeEncoding ClbS from Samsonia erythrinae DSM 16730DepositorInsertEncoding ClbS from Samsonia erythrinae DSM 16730 (Codon optimized for E coli)
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc-clbS_Gibsiella
Plasmid#223356PurposeEncoding ClbS from Gibbsiella quercinecans DSM 25889DepositorInsertEncoding ClbS from Gibbsiella quercinecans DSM 25889 (Codon optimized for E coli)
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_zCas9
Plasmid#213913PurposeEntry clone containing 3×FLAG-NLS-zCas9-NLS encoding sequence, Pisum sativum rbcS E9 terminator. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertzCas9
UseCRISPRTagsExpressionMutationZea mays codon-optimized Cas9PromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_CHHF_GPC
Plasmid#207260PurposeMammalian expression of Chapare hemorrhagic fever virus GPC proteinDepositorInsertCHHF_GPC (CVsS_gp1 Chapare hemorrhagic fever virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Atg11 [699-800] pHis2
Plasmid#190264PurposeCodon optimized Atg11 amino acids 699-800 from S. cerevisiae for expression in E. coli.DepositorInsertATG11
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
VPS13D1-1576-(Y768A)
Plasmid#194006PurposeY to A mutation in VPS13D geneDepositorInsertCodon optimized VPS13D (VPS13D Human)
UseTagsnoneExpressionMammalianMutationPromoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
VPS13D1-1576-(Y1253S)
Plasmid#194005PurposeY to S mutation in VPS13D geneDepositorInsertCodon optimized VPS13D (VPS13D Human)
UseTagsnoneExpressionMammalianMutationPromoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g1_CASH-1
Plasmid#188975PurposeA human codon-optimized SpCas9 nickase and chimeric g1 guide RNA expression plasmid.DepositorInsertg1 guide RNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1
Plasmid#188976PurposeA human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.DepositorInsertg2 guide RNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJF152_Phsp_Cas9
Plasmid#163862Purposeheat shock-induced expression of Cas9 in C. elegansDepositorInsertCas9, C. elegans codon optimized with intron and SV40_NLS
UseCRISPRTagsExpressionWormMutationPromoterPhsp-16.48Available sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-HypaCAS9-pA
Plasmid#131467PurposeExpresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized HypaCas9
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
p1146-pCSDest-AcrIIC3Nme-mCherry
Plasmid#126202PurposeMammalian expression of AcrIIC3Nme fused to mCherryDepositorInsertcodon-optimized AcrIIC3Nme
UseTagsFLAG/NLS and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTN177E-myc
Plasmid#107474PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation N177E with approximately wildtype activityDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationR596QPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only