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Plasmid#196987PurposeFor SECRETS protocol to screen for gRNA activity and specificity: BsaI cassette for gRNA expression or for cloning (x-)gRNA librarys + off-target for SECRETS counter-selection.DepositorInsertSpy gRNA cassette
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpltetO1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMB2a-TelR
Plasmid#204985PurposeLevel 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB2b-TelR
Plasmid#204986PurposeLevel 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB2-A-TelR
Plasmid#204987PurposeLevel 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB3-A-TelR
Plasmid#204991PurposeLevel 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505:GFP
Plasmid#193001PurposePlasmid to generate a transgenic Hydra Zic4 reporter lineDepositorInsertHydra Zic4 promoter
UseHydra transgenesis vectorTagsGFPExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
shRNA-Ratio-PosCon
Plasmid#183553PurposeDesigned to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio positive control (Pbrm1), Figure S2)DepositorInsertPbrm1 shRNA
UseTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
shRNA-Ratio-NegCon
Plasmid#183554PurposeDesigned to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio negative control (Luciferase), Fig S2)DepositorInsertLuciferase shRNA
UseTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJo55
Plasmid#159623PurposeAn empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
MYMH 391
Plasmid#138059PurposeEncodes Anderson promoter variant using mRFP as a reporter geneDepositorInsertJ23101- pSEVA-331Bb- RFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 392
Plasmid#138053PurposeEncodes Anderson promoter variant using mRFP as a reporter geneDepositorInsertJ23102- pSEVA-331Bb- RFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 406
Plasmid#138067PurposeEncodes Anderson promoter variant using mRFP as a reporter geneDepositorInsertJ23100- pSEVA-331Bb- RFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 405
Plasmid#138066PurposeEncodes Anderson promoter variant using mRFP as a reporter geneDepositorInsertJ23119- pSEVA-331Bb- RFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 398
Plasmid#138065PurposeA novel variant of the Anderson promoter using mRFP as a reporter geneDepositorInsertJ23101-3TG- pSEVA-331Bb- RFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 397
Plasmid#138064PurposeEncodes Anderson promoter variant using mRFP as a reporter geneDepositorInsertJ23115- pSEVA-331Bb- RFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 396
Plasmid#138063PurposeEncodes Anderson promoter variant using mRFP as a reporter geneDepositorInsertJ23110- pSEVA-331Bb- RFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 395
Plasmid#138062PurposeEncodes Anderson promoter variant using mRFP as a reporter geneDepositorInsertJ23109- pSEVA-331Bb- RFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 394
Plasmid#138061PurposeEncodes Anderson promoter variant using mRFP as a reporter geneDepositorInsertJ23108- pSEVA-331Bb- RFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 393
Plasmid#138060PurposeEncodes Anderson promoter variant using mRFP as a reporter geneDepositorInsertJ23105- pSEVA-331Bb- RFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEYRC-D1A
Plasmid#127753PurposeResearch the effect of gene orientation of transcriptionDepositorInsertRFP, CFP, Divergent Orientation
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only