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pFRT/FLAG/HA-DEST EIF2C2

Plasmid

#19888

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

Eukaryotic translation initiation factor 2C, 2 (AGO2 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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RH5-bio

Plasmid

#47780

Purpose

Expresses enzymatically monobiotinylated full-length RH5 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised RH5

Use

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2-hDKK2-flag

Plasmid

#15495

Depositor

Sergei Sokol

Article

Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Insert

DKK2 (DKK2 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-GRAB_OTmut

Plasmid

#185388

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Has Service

AAV9

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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MoFGFR2b-ires neo

Plasmid

#86491

Purpose

mamalian cell expression of FGFR2b

Depositor

David Ornitz

Article

Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

Insert

FgfrR2 IIIb 13A4 (FGFR2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Moloney murine leukemia virus LTR

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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p5071 pcDNA4c hBrd4 (595-1362)

Plasmid

#14450

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (595-1362) (BRD4 Human)

Use

Tags

Expression

Mammalian

Mutation

amino acids 595-1362

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pET15b-Casp8 delta DED C360A

Plasmid

#11828

Depositor

Guy Salvesen

Article

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 8 minus Death Effecter Domain catalytic mutant (CASP8 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Minus Death Defecter Domain (aa 1-216), C360A

Promoter

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
pENTR4_CELF1

Plasmid

#106098

Purpose

Entry vector for CELF1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

CELF1 (CELF1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Casp8

Plasmid

#48172

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of CASP8 (CASP8 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
bdSUMO-TAT-GFP-HiBit

Plasmid

#170991

Purpose

For bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-TAT-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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LSS_pCSdest

Plasmid

#53931

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

LSS (LSS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
p5070 pcDNA4c hBrd4 (1047-1362)

Plasmid

#14449

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1047-1362) (BRD4 Human)

Use

Tags

Expression

Mammalian

Mutation

amino acids 1047-1362 of Brd4 (the CTD)

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pUHD.NFATc1nuc

Plasmid

#40616

Depositor

Jerry Crabtree

Article

Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

Insert

NFATc1nuc (NFATC1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S172A S175A S176A S178A S179A S181A S184A S187A S…

Promoter

CMV2

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti6-BMPR1A(Q233D)-V5

Plasmid

#35638

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

BMP Receptor Type1A Q233D (BMPR1A Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Glutamine 233 to Aspartate

Promoter

CMV

Available since

April 26, 2012

Availability

Academic Institutions and Nonprofits only
Stat1 beta pRc/CMV

Plasmid

#8703

Depositor

Jim Darnell

Article

Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

Insert

STAT1 beta (STAT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2005

Availability

Academic Institutions and Nonprofits only
GA-MFF

Plasmid

#141160

Purpose

Dimerization-dependent green fluorescent protein targeted to mitochondrial outer membrane

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

mitochondrial fission factor (MFF Human)

Use

Tags

GA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ266

Plasmid

#164713

Purpose

Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

zCas9(D10A)-PmCDA1-UGI

Use

CRISPR; Gateway compatible pmcda1-cbe_v01 entry c…

Tags

Expression

Plant

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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CAV1-K*R-mEGFP

Plasmid

#27766

Depositor

Ari Helenius

Article

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Insert

caveolin-1, lysine null mutant (CAV1 Human)

Use

Tags

monomeric EGFP

Expression

Mammalian

Mutation

All lysines mutated to arginines (K*R)

Promoter

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

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p5069 pcDNA4c hBrd4 (471-730)

Plasmid

#14448

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (471-730) (BRD4 Human)

Use

Tags

Expression

Mammalian

Mutation

amino acids 471-730

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pLC242-GFP-metap2

Plasmid

#100518

Purpose

Expresses GFP tagged metap2 in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

Metap2 (METAP2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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