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Plasmid#74223PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsMyc and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only
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pET28:GFP
Plasmid#60733PurposepET28 driving expression of GFP, biobrick part Bba_E0040DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-N1
Plasmid#54590PurposeLocalization: N1 Cloning Vector, Excitation: 604, Emission: 659DepositorTypeEmpty backboneUseTagsmCardinalExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRK5 HA-Nprl2
Plasmid#99709PurposeMammalian expression of HA-Nprl2DepositorInsertNprl2 (NPRL2 )
UseTagsHAExpressionMutationPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-VAPB
Plasmid#108126PurposeER markerDepositorInsertmCherry:VAPB (VAPB Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Clover
Plasmid#40259PurposeGFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlapDepositorHas ServiceCloning Grade DNAInsertClover GFP
UseTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast
Plasmid#205565PurposeExpress human NEDD4L (delta C2 isoform)DepositorInsertNEDD4L (NEDD4L Human)
UsePiggybacTagsExpressionMammalianMutationdelta C2 isoformPromoterEF1Available sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSP3535
Plasmid#46886PurposeGram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible ExpressionDepositorTypeEmpty backboneUseNisin-inducible expression; gram-positive bacteri…TagsExpressionBacterialMutationPromoterPnisAAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYPQ203 (pMDC32-Ubi1)
Plasmid#86207PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance markerDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorTagsExpressionPlantMutationPromoterMaize ubiquitin 1 (ZmUbi1)Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbALFA
Plasmid#184678PurposeNIR-Fb to ALFA-tagDepositorInsertNIR-FbALFA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_L_EBOV
Plasmid#103052PurposeEncodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertL gene of Ebola virus (L Zaire ebolavirus, Mayinga)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
4xmts-mNeonGreen
Plasmid#98876PurposeIn vivo visualization of mitochondria (can be used for colocalization studies)DepositorArticleInsert4xmts
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRRL Luciferase
Plasmid#120798PurposeFor production of lentivirus to express luciferase, primarily for in vivo bioluminescence tracking experiments.DepositorInsertLuciferase
UseLentiviralTagsExpressionMammalianMutationPromoterhPGKAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF3485
Plasmid#144961PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertATF2 (ATF2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEJS578_DD-dSpyCas9-mCherry-APEX2
Plasmid#108570PurposeExpression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factorsDepositorInsertdSpyCas9
UseCRISPR and LentiviralTagsAPEX2, Destabilization domains, Flag, and mCherry…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_VP30_EBOV
Plasmid#103051PurposeEncodes Ebola virus VP30 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertVP30 gene of Ebola virus (VP30 Zaire ebolavirus, Mayinga)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCCL-CellCycle
Plasmid#132429PurposeExpressing Fucci cell cycle indicator in mammalian cellsDepositorInserthCdt, hGeminin
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DEST47 PINK1 C-GFP
Plasmid#13316DepositorInsertPINK1 (PINK1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRC0940 (circRNA entry vector)
Plasmid#189967PurposeCloning vector for generating template plasmids to transcribe circRNA, with mNeonGreen dropout following BsaI digestionDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
HRH3-Tango
Plasmid#66402PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertHRH3 (HRH3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Px461-Cas9n-Trp53-sgRNA-beta
Plasmid#88847PurposeCRISPR KO of Trp53DepositorInsertTrp53 (Trp53 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-Nme2ABE8e
Plasmid#189928PurposePlasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.DepositorInsertNme2ABE8e
UseTagsNLSExpressionMammalianMutationNme2Cas9 D16APromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Red Glifon 3000
Plasmid#163116PurposeRed fluorescent protein-based glucose sensor for mammalian cells with lower affinityDepositorInsertRed Glifon 3000
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMALB-ATF4.5
Plasmid#155032PurposeFluorescent reporter for ATF4 translationDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-sp-sgRNA-RNF2_+41nick
Plasmid#135958PurposeS. pyogenes sgRNA for +41 nick in RNF2DepositorInsertRNF2_+41 nicking sgRNA
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ABCC1_STOP
Plasmid#221423PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertABCC1 (ABCC1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ABCB1_STOP
Plasmid#221424PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertABCB1 (ABCB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
miniTurbo-His6_pET21a
Plasmid#107178Purposeexpresses His6-tagged miniTurbo in bacteriaDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionBacterialMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-MLS-KillerRed
Plasmid#115517PurposeMiddle entry plasmid containing membrane bound KillerRed fluorophoreDepositorInsertMLS-KillerRed
UseGateway cloningTagsMLSExpressionMutationPromoterAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLGB31
Plasmid#126621PurposeSuicide vector for allelic replacement in Bacteroides, inulin selection and rhamnose-inducible ss-Bfe1 counterselectionDepositorTypeEmpty backboneUseAllellic exchange in bacteriaTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only