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Plasmid#110970PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertconserved Plasmodium protein (PF3D7_1462300 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSkipFlox
Plasmid#85797PurposeP. falciparum plasmid for expression of both diCre fragments from single casette using skip peptideDepositorInsertsplit Cre recombinase
UseCre/Lox; P. falciparum expressionTagsFKBP, FRB*ExpressionMutationPromoterPfcrtAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
p1xNLS-FRB-mCherry-hsp86-BSD
Plasmid#85795PurposeExpression of 1xNLS mislocaliser in P. falciparumDepositorInsert1xNLS Mislocaliser
UseP. falciparum expressionTagsFRB*, mCherryExpressionMutationPromoterPfhsp86Available sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLyn-FRB-mCherry-nmd3-BSD
Plasmid#85796PurposeExpression of PPM mislocaliser in P. falciparumDepositorInsertPPM Mislocaliser
UseP. falciparum expressionTagsFRB*, mCherryExpressionMutationPromoterPfnmd3Available sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR R4 ATCC LacZ GCTT
Plasmid#173668PurposeDestination vector for Golden Gate Cloning assemblies, blue-white screeningDepositorTypeEmpty backboneUseTagsExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
S. aureus DHPS
Plasmid#64079PurposeExpression of Staphylococcus aureus dihydropteroate synthaseDepositorInsertdihydropteroate synthase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_0910300-COMP-blac-flag-his
Plasmid#110959PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertconserved Plasmodium protein, unknown function (PF3D7_0910300 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_1351800.1-COMP-blac-flag-his
Plasmid#111009PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertconserved Plasmodium protein (PF3D7_1351800.1 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_0505700-COMP-blac-flag-his
Plasmid#110988PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertconserved Plasmodium membrane protein (PF3D7_0505700 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_1434400-COMP-blac-flag-his
Plasmid#110981PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertconserved Plasmodium membrane protein (PF3D7_1434400 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_1105300-COMP-blac-flag-his
Plasmid#110960PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertconserved Plasmodium protein, unknown function (PF3D7_1105300 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLI-2xFKBP-GFP(1463000)
Plasmid#85789PurposeP. falciparum integration plasmid to fuse endogenous genes with tags using SLIDepositorInsertPF3D7146300 targeting region, 2xFKBP-GFP-2TA-Neo-R
UseP. falciparum integrationTags2xFKBP, GFPExpressionMutationPromoternoneAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLI-sandwich(0807600)
Plasmid#85790PurposeP. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLIDepositorInsertPF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R
UseP. falciparum genomic integrationTags2xFKBP, GFPExpressionMutationPromoternoneAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLI-TGD(1463000)
Plasmid#85791PurposeP. falciparum integration plasmid to disrupt genes using SLIDepositorInsertPF3D7146300 targeting region, GFP-2TA-Neo-R
UseP. falciparum genomic integration (gene disruptio…TagsGFPExpressionMutationPromoternoneAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLI-N-GFP-2xFKBP-loxP(K13)
Plasmid#85792PurposeP. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLIDepositorInsertKelch13
UseCre/Lox; P. falciparum genomic integrationTags2xFKBP, GFPExpressionMutationPromoternoneAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
Prohibitin-bio-His
Plasmid#50800PurposeExpresses enzymatically monobiotinylated full length ProhibitinDepositorInsertProhibitin
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
GLURP-bio-His
Plasmid#50802PurposeExpresses enzymatically monobiotinylated full length GLURPDepositorInsertGLURP
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
SERA1-bio-His
Plasmid#50812PurposeExpresses enzymatically monobiotinylated full length SERA1DepositorInsertSERA1
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
SERA5-bio-His
Plasmid#50817PurposeExpresses enzymatically monobiotinylated full length SERA5DepositorInsertSERA5
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
SERA9-bio-His
Plasmid#50820PurposeExpresses enzymatically monobiotinylated full length SERA9DepositorInsertSERA9
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 12, 2014AvailabilityAcademic Institutions and Nonprofits only