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Plasmid#174661PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.DepositorInsertHfq
UseTagsalpha NTDExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pBRα-β flap (831-1057)
Plasmid#53734PurposeExpression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertalpha NTD-Beta flap fusion
UseTagsExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJin-278
Plasmid#125714PurposeABA-inducible split T7 GFP vector using RNAPN d5-19DepositorInsertPT7-GFP mRNA expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH462
Plasmid#172154PurposepylBCD variant in pBAD backboneDepositorInsertPara (Sumo-tagged pylBCD); AraC
UseTagsExpressionBacterialMutationSUMO tag fused to N-terminus of PylBPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEV_S
Plasmid#100266PurposepDSW204 carrying genes entABCDE, vibHF and a cos site, for serratiochelin and analogs production in E. coliDepositorInsertsUseSynthetic Biology; Expression, pbr322TagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-RTA
Plasmid#99675PurposeExpresses dSp Cas9 fused at C term. To VP64 and RTA activation domainsDepositorArticleInsertdCas9
UseTagsVP64-RTAExpressionMammalianMutationdead Cas9PromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pReg-ROTU4
Plasmid#190669PurposeExpresses an evolved rotundine biosensor in E. coliDepositorInsertROTU4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pReg-THP4
Plasmid#190671PurposeExpresses an evolved tetrahydropapaverine biosensor in E. coliDepositorInsertTHP4
UseSynthetic BiologyTagsExpressionMutationPromoterJ23103Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGfOMT1-GEN4
Plasmid#190612PurposeExpresses an evolved methyltransferase for tetrahydropapaverine biosynthesis in E. coliDepositorInsertGfOMT-GEN4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pReg-GLAU4
Plasmid#190667PurposeExpresses an evolved glaucine biosensor in E. coliDepositorInsertGLAU4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pReg-PAP4
Plasmid#190668PurposeExpresses an evolved papaverine biosensor in E. coliDepositorInsertPAP4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pReg-NOS4
Plasmid#190670PurposeExpresses an evolved noscapine biosensor in E. coliDepositorInsertNOS4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH577
Plasmid#172161PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 3f2); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E84K, E122K, E178K, G188R, E251D) PylD…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH26_pBad_AraC_Para-sfGFP.1TAG
Plasmid#163721PurposeArabinose-inducible expression of green fluorescent protein encoding one amber codonDepositorInsertaraC gfp.1TAG
UseTagsExpressionBacterialMutationgfp.N39TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH27_pBad_AraC_Para-sfGFP.2TAG
Plasmid#163722PurposeArabinose-inducible expression of green fluorescent protein encoding two amber codonsDepositorInsertaraC gfp.2TAG
UseTagsExpressionBacterialMutationgfp.N39TAG,Y151TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH807_pJH_LldR_Pcon-sfGFP.2TAG
Plasmid#163738PurposeConstitutive expression of green fluorescent protein encoding two stop codonsDepositorInsertlldr gfp.2TAG
UseTagsExpressionBacterialMutationgfp.N39TAG,Y135TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH809_pJH_LldR_Pcon-sfGFP.10TAG
Plasmid#163739PurposeConstitutive expression of green fluorescent protein encoding ten stop codonsDepositorInsertlldr gfp.10TAG
UseTagsExpressionBacterialMutationgfp with first 10 leucine codons mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRF032d
Plasmid#156353PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyTagsExpressionMutationWT antiRBS engineered to orthogonal antiRBS, PS e…PromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PJ14
Plasmid#25912DepositorInsertIg germline mu switch region
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only