Addgene

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TVBB N-term-mScarlet

Plasmid

#169218

Purpose

Targeting vector backbone to support a knock-in of mScarlet-Linker at the N-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mScarlet

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLBS.CAG/NLS-mScarlet

Plasmid

#129336

Purpose

nucleus marker, lentivirus

Depositor

Yasushi Okada

Insert

mScarlet (Addgene 85042)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only
pCAX-mScarlet-FBP17

Plasmid

#178366

Purpose

Expresses FBP17 c-terminal labeled with mScarelt in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Formin Binding Protein 17 (FNBP1 Human)

Use

Tags

Expression

Mammalian

Mutation

400 Glutamic Acid changed to Aspartic acid (403 A…

Promoter

Chicken Beta Actin

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMHE-Pom121-mScarlet

Plasmid

#171491

Purpose

T7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

Pom121 (Pom121 Mouse)

Use

Pgehme

Tags

mScarlet

Expression

Mutation

Promoter

T7

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 AIF-mScarlet

Plasmid

#129576

Purpose

Expression of mScarlet fused AIF

Depositor

Stephen Tait

Article

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

AIF (AIFM1 Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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YIplac211-Sec7-mScarlet2Ix6

Plasmid

#140151

Purpose

Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-mScarlet2Ix6

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

SEC7

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mScarlet

Plasmid

#179818

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

mScarlet

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_mGFP-FGFR3-mScarlet

Plasmid

#192509

Purpose

Expression of N-terminally mGFP and C-terminally mScarlet labelled wildtype FGFR3 (isoform IIIc)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc-mScarlet

Use

Tags

mGFP and mScarlet

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAT9752-BEAR-mScarlet

Plasmid

#162991

Purpose

BEAR target plasmid with split mScarlet and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mScarlet split with an intron between amino acids 110-111

Use

CRISPR

Tags

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-ER [TM(SAC1)]

Plasmid

#186572

Purpose

Expression of the Endoplasmic Reticulum marker mScarlet-ER [TM(SAC1)] in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

SAC1 (SACM1L Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C-terminal transmembrane region of the protein: 5…

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pIVTR(T7A)-mScarlet

Plasmid

#172294

Purpose

In vitro transcription of mScarlet red fluorescent protein

Depositor

Patrik Verstreken

Insert

mScarlet

Use

Other

Tags

Expression

Mutation

Promoter

T7 promoter A-initiating

Available since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_CKI(0.4)_HA_AzPPO_AgeI_nrxn1a_WT_IRES_mScarlet

Plasmid

#205289

Purpose

Bistable opsin for neuronal inhibition

Depositor

Matthias Prigge

Insert

Parapinopsin

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pQE-60NA-mScarlet-2I

Plasmid

#160463

Purpose

Encodes a variant of mScarlet-I that displays reduced cytotoxicity in bacteria

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

mScarlet-2I

Use

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mScarletI

Plasmid

#201442

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

mScarlet-I

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Double UP sfGFP to mScarlet

Plasmid

#120261

Purpose

Natively produces sfGFP, in the presence of Cre will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Insert

sfGFP/mScarletP2A-mcs

Use

Tags

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Promoter

CAG

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pKUNOC-ErbB1-mScarlet

Plasmid

#197360

Purpose

Encoding human ErbB1 tagged with mScarlet.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

ERBB1 (EGFR Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAX-mScarlet-CIP4

Plasmid

#178364

Purpose

Expresses CIP4 c-terminal labeled with mScarlet in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Cdc42 Interacting Protein 4 (TRIP10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Double UP mClover3 to mScarlet

Plasmid

#120262

Purpose

Natively produces mClover3, in the presence of Cre will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Insert

mClover3/mScarletP2A-mcs

Use

Tags

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Promoter

CAG

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-mScarletI

Plasmid

#179820

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

mScarlet-I

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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Double UP Halotag to mScarlet

Plasmid

#125138

Purpose

Natively produces Halotag, in the presence of Cre will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge