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Showing: 201 - 220 of 230 results

  3. mCit-4aa-SNAP25B

    Plasmid
    #53235
    Purpose
    Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    SNAP-25B (Snap25 Rat)
    Use
    Tags
    4aa-linker and mCitrine
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    53235-plasmid-map-sequence-id-421058

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  7. JD179

    Plasmid
    #229496
    Purpose
    Adenoviral helper plasmid containing inducible E2A and L4-22k/33k genes and constitutive VA RNA I gene
    Depositor
    Nerea Zabaleta
    Article
    Doshi et al Molecular Therapy Methods & Clinical Dev
    Insert
    E2A, L4-22k/33k, VA RNA I
    Use
    AAV and Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tre (E2A, L4-22k/33k). Wt promoter (VA RNA I), ET…
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    229496-plasmid-map-sequence-id-455847

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  8. mCit-APPAP-gs

    Plasmid
    #228090
    Purpose
    Mammalian expression of five coiled coil segments in APPAP arrangment, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, APPAP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228090-plasmid-map-sequence-id-451134

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  9. mCit-APPAPAPPAP-gs

    Plasmid
    #228091
    Purpose
    Mammalian expression of 10 coiled coil segments in APPAPAPPAP arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, APPAPAPPAP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    228091-plasmid-map-sequence-id-451163

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  10. VAMP2-12aa-mCit

    Plasmid
    #53237
    Purpose
    Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    VAMP2 (Vamp2 Rat)
    Use
    Tags
    12aa-linker and mCitrine
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. RFP-PPPPPPAAAA-gs

    Plasmid
    #228092
    Purpose
    Mammalian expression of 10 coiled coil segments in PPPPPPAAAA arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, PPPPPPAAAA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    228092-plasmid-map-sequence-id-451390

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  12. mCit(S1h)8-gs

    Plasmid
    #228084
    Purpose
    Mammalian expression of coiled coil segments (S1h)x8, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, S1h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228084-plasmid-map-sequence-id-451170

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  13. mCit(P5f-P13f)3-gs

    Plasmid
    #228080
    Purpose
    Mammalian expression of coiled coil segments (P5f-P13f)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, P5f, P13f
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    228080-plasmid-map-sequence-id-454693

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  14. mCit(S1h)3(S3h)3-gs

    Plasmid
    #228079
    Purpose
    Mammalian expression of coiled coil segments (S1h)x3-(S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, S1h, S3h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    228079-plasmid-map-sequence-id-454689

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  15. mCit(S1h-S3h)3-gs

    Plasmid
    #228076
    Purpose
    Mammalian expression of coiled coil segments (S1h-S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, S1h, S3h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    228076-plasmid-map-sequence-id-454655

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  16. mCit(D1h-D3h)3-gs

    Plasmid
    #228088
    Purpose
    Mammalian expression of coiled coil segments (D1h-D3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, D1h, D3h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    228088-plasmid-map-sequence-id-451399

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  17. pGEM-PH-citrine

    Plasmid
    #131406
    Purpose
    Purpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrine
    Depositor
    Lars Hufnagel
    Article
    Guignard et al Science. 2020 Jul 10;369(6500). pii: 369
    Insert
    PH
    Use
    In vitro transcription
    Tags
    citrine yellow fluorescent protein
    Expression
    Mutation
    Promoter
    T7
    Available since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131406-plasmid-map-sequence-id-261478

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Showing: 201 - 220 of 230 results