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Plasmid#188311PurposeExpression of Firefly luciferase via a T7 promoter in TXTLDepositorInsertFirefly luciferase
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-RLuc
Plasmid#188312PurposeExpression of Renilla luciferase via a T7 promoter in TXTLDepositorInsertRenilla luciferase
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular
Plasmid#132962PurposeFirefly-luciferase single-strand annealing assay cloning backboneDepositorInsertFirefly Luciferase
UseCRISPR and LuciferaseTagsExpressionMammalianMutationStop sequence between luciferase genePromoterCMVAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUPD+Red-F
Plasmid#170874PurposePhytobrick - Red-F Luciferase CDSDepositorInsertRed-F
UseLuciferase and Synthetic BiologyTagsExpressionMutationp.S286Y Red mutantPromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pomMx1
Plasmid#30536PurposeReporter (luciferase) for interferon signalling in fishDepositorInsertrainbow trout Mx 1 promoter
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGreen2x35SLUC
Plasmid#108228PurposePlant expression as translational fusions to FLUC with RLUC normalizationDepositorTypeEmpty backboneUseLuciferaseTagsFirefly LuciferaseExpressionPlantMutationPromoter2x35SAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
FL
Plasmid#120522PurposeFirefly luciferase reporterDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
LRTi
Plasmid#214072PurposeLuminescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LRTX_ctrl
Plasmid#214073PurposeLuminescent reporter system, NMD negative control. No intron in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationRemnant of linker sequence present at the end of …PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LRT_RTctrl
Plasmid#214074PurposeLuminescent reporter system, readthrough control, NMD negative control. No intron in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LRT
Plasmid#214071PurposeLuminescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRTX_ctrl
Plasmid#214077PurposeLuminescent reporter system, NMD negative control. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationRemnant of linker sequence present at the end of …PromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
MLRV
Plasmid#118069PurposeMulti-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reportersDepositorInsertsTB:5xNF-κβ:RedF:bGHpA
TB:4xTGF-β:FLuc:bGHpA
TB:5xE-box:Renilla:bGHpA
TB:2xp53:NLuc:bGHpA
TB:6xAP-1:GrRenilla:bGHpA
CMV:ELuc:bGHpA
UseLuciferase and Synthetic Biology; Assembly of gb2…TagsExpressionMammalianMutationPromoter2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…Available sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-mutLuc
Plasmid#106458Purposeluciferase reporter, CMV promoter (3 NFkB sites mutated)DepositorInsertluciferase reporter, CMV promoter (3 NFkB sites mutated)
UseLuciferaseTagsExpressionMammalianMutation3 NFkB sites mutatedPromoterAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+E-Luc
Plasmid#170873PurposePhytobrick - E-Luc Luciferase CDSDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionMutationc.375C>T to remove BbsI sitePromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
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LiRT
Plasmid#214075PurposeLuminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRT_RTctrl
Plasmid#214078PurposeLuminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRTi
Plasmid#214076PurposeLuminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB1a2R+pNOS_Red-F_NOSt
Plasmid#170888PurposeGoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Reverse orientationDepositorInsertRed-F
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationp.S286Y Red mutantPromoterpNOSAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only