-
Plasmid#72901PurposeEarly endosome-targeted mCherry-tagged recruiter constructDepositorInsertRab5a (RAB5A Human)
UseLentiviralTagsFKBP(F36V) and mCherryExpressionMammalianMutationfragment a.a. 2-215PromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-sNRR-T2A-BFP
Plasmid#194930PurposeLV construct encoding sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-NRR-T2A-BFP
Plasmid#194929PurposeLV construct encoding NRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-NRR-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-Y186F-T2A-BFP
Plasmid#194931PurposeLV construct encoding Y186F mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(Y186F)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-Y186A-T2A-BFP
Plasmid#194932PurposeLV construct encoding Y186A mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-FKBP:mCherry-BclXLc33
Plasmid#72905PurposeOuter mitochondrial membrane-targeted mCherry-tagged recruiter constructDepositorInsertBl2-like protein 1, isoform Bcl-X(L) (fragment)
UseLentiviralTagsFKBP, mCherryExpressionMammalianMutation33 C-terminal a.a.PromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-R103A-T2A-BFP
Plasmid#194935PurposeLV construct encoding R103A mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(R103A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV TRE-GFP-TMD-DLL1
Plasmid#194936PurposeLV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.DepositorInserteGFP-(PDGFR)TMD-rDLL1 ICD (WT)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
GESTALT_pX330-v1
Plasmid#103061Purposeplasmid px330 with guide targeting GESTALT v1 through V5 constructsDepositorInsertintegration of Cas9 with guide targeting GESTALT barcodes V1 to V5
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-Y192A-T2A-BFP
Plasmid#194933PurposeLV construct encoding Y192A mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(Y192A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
TetOn-mCherry-eGFP-RAMP4
Plasmid#109014PurposeTet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)DepositorInsertRAMP4 (SERP1 Human)
UseLentiviralTagsmCherry-eGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX304-Flag-APEX2-NES
Plasmid#92158PurposeCytosolic APEX2 constructDepositorInsertFlag-APEX2-NES
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-hGtACR1-EYFP
Plasmid#67795PurposeMammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backboneDepositorInserthGtACR1
UseLentiviralTagsEYFPExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-hGtACR2-EYFP
Plasmid#67877PurposeMammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backboneDepositorInserthGtACR2
UseLentiviralTagsEYFPExpressionMammalianMutationPromoterUbCAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
LZF40: hSyn-Cre
Plasmid#104124PurposesynOptopatch constructDepositorInsertCre
UseLentiviralTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP
Plasmid#194934PurposeLV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(Y186F/Y229A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-pNFkB::Cre-mRuby3
Plasmid#164137PurposeLentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFaDepositorInsertNFkB promoter-Cre-T2A-mRuby3
UseCRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…TagsExpressionMammalianMutationPromoterNFkB synthetic promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh-Puro
Plasmid#31845PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherry-puromycinAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
lentiTet-LDLR-mCherry BlastR
Plasmid#186739PurposeDox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertLDLR (LDLR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-pHSE::Cre-mRuby3
Plasmid#164136PurposeLentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat inductionDepositorInsertHSE promoter-Cre-T2A-mRuby3
UseCRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…TagsExpressionMammalianMutationPromoterHSE synthetic promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only