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GNAS_pLENTI-CAG-IRES-GFP

Plasmid

#176991

Purpose

Mammalian lentiviral expression vector encoding GNAS

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GNAS (GNAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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APP_pcDNA6.2/EmGFP-Bsd

Plasmid

#176954

Purpose

Mammalian expression vector encoding APP and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

APP (APP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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GNAS_pcDNA6.2/EmGFP-Bsd

Plasmid

#176976

Purpose

Mammalian expression vector encoding GNAS and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GNAS (GNAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

Plasmid

#51248

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907,980,1006 mutated to A…

Promoter

EF1a

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only
CHAF1B_pcDNA6.2/EmGFP-Bsd

Plasmid

#176969

Purpose

Mammalian expression vector encoding CHAF1B and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

CHAF1B (CHAF1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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B3GALT5_pLENTI-CAG-IRES-GFP

Plasmid

#177006

Purpose

Mammalian lentiviral expression vector encoding B3GALT5

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

B3GALT5 (B3GALT5 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

Plasmid

#51257

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248,789,805,817,848,9…

Promoter

EF1a

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
CHAF1B_pLENTI-CAG-IRES-GFP

Plasmid

#177009

Purpose

Mammalian lentiviral expression vector encoding CHAF1B

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

CHAF1B (CHAF1B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
BACE2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176942

Purpose

Mammalian expression vector encoding BACE2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

BACE2 (BACE2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A

Plasmid

#51249

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 980,1006 mutated to Ala

Promoter

EF1a

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only
GP805

Plasmid

#164545

Purpose

Expresses Smo-FLAG K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

All 21 cytoplamic Lysines of Smoothened changed t…

Promoter

GgCryD1

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A

Plasmid

#51247

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907 mutated to Ala

Promoter

EF1a

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
JAM2_pLENTI-CAG-IRES-GFP

Plasmid

#176997

Purpose

Mammalian lentiviral expression vector encoding JAM2

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

JAM2 (JAM2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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CILK1-R272Q

Plasmid

#214911

Purpose

expression of the mutant variant of CILK1

Depositor

Pavel Krejčí

Article

Paige Taylor et al Hum Mol Genet. 2016 Sep 15;25(18):3998-4

Insert

CILK1 (CILK1 Human)

Use

Tags

Myc-FLAG

Expression

Mammalian

Mutation

R272Q substitution

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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CILK1-E80K

Plasmid

#214910

Purpose

expression of the mutant variant of CILK1

Depositor

Pavel Krejčí

Article

Paige Taylor et al Hum Mol Genet. 2016 Sep 15;25(18):3998-4

Insert

CILK1 (CILK1 Human)

Use

Tags

Myc-FLAG

Expression

Mammalian

Mutation

E80K substitution

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE44

Plasmid

#90382

Purpose

Hedgehog - Gli1 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

RUNX

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAct5C-Hh

Plasmid

#37382

Depositor

Norbert Perrimon

Article

Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

Insert

hedgehog (hh Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

actin5C

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pIhh-5A9-LUC

Plasmid

#46318

Depositor

Xiangli Yang

Article

Wang et al Development. 2009 Dec;136(24):4143-53. d

Insert

5 copies of A9 sequence from Ihh promoter

Use

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

mouse osteocalcin gene 2 promoter

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
7Gli:GFP

Plasmid

#110494

Purpose

reporter of Hedgehog signaling activity

Depositor

Michael Lewis

Article

Li et al PLoS Genet. 2016 May 19;12(5):e1006054.

Insert

7 repeats of the Gli binding site GAACACCCA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

7 repeats of Gli biding site

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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OA-1045B

Plasmid

#125004

Purpose

Expresses gRNAs targeting hid, eve, and hedgehog

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

U6:3

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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