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Flag-Tagged Clavesin1 (pCMV-Tag2B-Sec14a)

Plasmid

#47430

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin1 (CLVS1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
GFP-Clavesin2 (pEGFP-C1-Sec14b)

Plasmid

#47420

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin2 (CLVS2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
GFP-CHC17KDP

Plasmid

#59799

Purpose

Expresses knockdown-proof human CHC17 tagged with GFP.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

CHC17 (CLTC Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Silent mutations in amino acids 61-65 to confer s…

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAMBIA-FLS2-myc-mCherry

Plasmid

#86159

Purpose

FLS2-mCherry expression

Depositor

Silke Robatzek

Article

Mbengue et al Proc Natl Acad Sci U S A. 2016 Sep 27;11

Insert

FLS2 (FLS2 Mustard Weed)

Use

Tags

mCherry and myc

Expression

Plant

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-TACC3KDP-shTACC3

Plasmid

#59356

Purpose

Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Use

RNAi

Tags

GFP

Expression

Mammalian

Mutation

Silent mutations in amino acids 30-33 to confer s…

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPC1-FLAG-Synaptojanin 1-145

Plasmid

#22293

Depositor

Pietro De Camilli

Article

Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

Insert

Synaptojanin 1_145 (SYNJ1 Human)

Use

Tags

Flag and GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPC1-FLAG-Synaptojanin 1-170

Plasmid

#22294

Depositor

Pietro De Camilli

Article

Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

Insert

Synaptojanin 1_170 (SYNJ1 Human)

Use

Tags

Flag and GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pEGFPC1-EPSIN 2

Plasmid

#22242

Depositor

Pietro De Camilli

Article

Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

Insert

Epsin 2 (EPN2 Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only
pBrain-GFP-FKBP-TACC3-shTACC3

Plasmid

#59354

Purpose

Knocks down endogenous TACC3 and expresses RNAi-resistant human TACC3 tagged with GFP & FKBP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Use

RNAi

Tags

EGFP and FKBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
CD8-pTagRFP657-LOVpep (T406A, T407A, I532A)

Plasmid

#100742

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CD8A (CD8A Human)

Use

Tags

LOVpep and tagRFP657

Expression

Mammalian

Mutation

Truncated

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-Epsin 2

Plasmid

#22241

Depositor

Pietro De Camilli

Article

Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

Insert

Epsin 2 (EPN2 Human)

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only
GST-beta2(616-951)

Plasmid

#100743

Purpose

Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Long isoform

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Dok1-EGFP

Plasmid

#174194

Purpose

Expresses Dok1 in mammalian cells

Depositor

Cheng-han Yu

Article

Yu et al Nat Commun. 2015 Oct 28;6:8672. doi: 10.

Insert

DOK1 (DOK1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCAS9n(BB)-2A-puro DNM1, A

Plasmid

#107795

Purpose

gRNA vector for knock-in gene editing at human DNM1 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

gRNA targeting human DNM1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCAS9n(BB)-2A-puro DNM1, B

Plasmid

#107796

Purpose

gRNA vector for knock-in gene editing at human DNM1 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

gRNA targeting human DNM1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CD8-dCherry-FRB

Plasmid

#100739

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis (dark mCherry K70N)

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CD8A (CD8A Human)

Use

Tags

FRB and dCherry

Expression

Mammalian

Mutation

Dark mCherry (K70N) non-fluorescent

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-Clta

Plasmid

#109585

Purpose

Multisite gateway vector for 3' tagging with Clathrin light chain a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Clathrin light chain a (clta Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-Cltb

Plasmid

#109586

Purpose

Multisite gateway vector for 3' tagging with Clathrin light chain b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Clathrin light chain b (cltb Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-beta2(616-951)Y815A

Plasmid

#100744

Purpose

Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Long isoform with Y815A mutation

Promoter

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
VAMP7 HA-delta pMEP4

Plasmid

#62958

Purpose

full length human VAMP7 with C-terminal HA tag cloned into delta pMEP4

Depositor

Paul Luzio

Article

Pryor et al Cell. 2008 Sep 5;134(5):817-27. doi: 10.

Insert

VAMP7 (VAMP7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only