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Plasmid#55354PurposeLocalization: Lysosomes, Excitation: 433, Emission: 475DepositorInsertCathespinB
UseTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMCSG49-AlleyCat7
Plasmid#42591DepositorInsertmutated C-terminal portion of calmodulin gene (M76-K148)
UseTagsHis and TEVExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Isole…PromoterT7Available sinceMarch 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AP-SpyCatcher
Plasmid#223636PurposeProtein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis10, AP fragment of split APEX2 tagExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiMutB3GALT5catdead-blast
Plasmid#220869Purposelentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequenceDepositorInsertbeta-1,3-galactosyltransferase 5 (B3GALT5 Human)
UseLentiviralTagsmyc, FLAGExpressionMammalianMutationencodes D156A and D243A mutations; also, nucleoti…PromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAT.482
Plasmid#203952PurposeSpectinomycin-based RSF1010 acceptor Level TDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_158 [TcatP]
Plasmid#210680PurposeLevel 0 partDepositorInsertTcatP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_157 [catP]
Plasmid#210679PurposeLevel 0 partDepositorInsertcatP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_156 [PcatP]
Plasmid#210678PurposeLevel 0 partDepositorInsertPcatP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP786_SpyCatcher-EZH2-FL-CO
Plasmid#211779PurposeSpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selectionDepositorInsertaGCN4-KRAB
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect KRAB e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP787_SnoopCatcher-EZH2-FL-CO
Plasmid#211780PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selectionDepositorInsertaGCN4-DNMT3A
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP780_SpyCatcher-KRAB-CO
Plasmid#211773PurposeSpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selectionDepositorInsertSpyCatcher-KRAB
UseTags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect KRAB e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP784_SnoopCatcher-DNMT3A-CO
Plasmid#211777PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertSpy-EZH2
UseTags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP781_SnoopCatcher-KRAB-CO
Plasmid#211774PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selectionDepositorInsertTraptavidin-KRAB
UseTags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect KRAB e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dox-SOScat
Plasmid#207329PurposePiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)DepositorInsertson of sevenless homolog 2 variant, catalytic domain
UseTagsmyristoylation tag - BFPExpressionBacterialMutationrange: 593 to 1078PromoterTet Response ElementAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_53 [TcatA]
Plasmid#198966PurposeLevel 0 partDepositorInsertTcatA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_52 [catA]
Plasmid#198965PurposeLevel 0 partDepositorInsertcatA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_32 [Pcat]
Plasmid#198945PurposeLevel 0 partDepositorInsertPcat
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_50 [catE]
Plasmid#198963PurposeLevel 0 partDepositorInsertcatE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_51 [PcatA]
Plasmid#198964PurposeLevel 0 partDepositorInsertPcatA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVY-14 TC-CAT
Plasmid#194628Purposeantibiotic resistance enzyme with tetracysteine tag for visualization of enzymeDepositorInserttetracysteine-CAT
UseTagsMGCCPGCCGGAExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only