Addgene

We narrowed to 599 results for: ca2

Showing: 201 - 220 of 599 results

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pTH1-SaSrtA-A

Plasmid

#64979

Purpose

Expresses the E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Use

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E108A

Promoter

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-K

Plasmid

#64978

Purpose

Expresses the E105K mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Use

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K

Promoter

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-D1ER

Plasmid

#36325

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1ER second generation cameleon (calcium sensor) targeted to ER

Use

Tags

KDEL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-4mtD3cpv

Plasmid

#36324

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria

Use

Tags

First 29 aa of human COX subunit 8a

Expression

Mutation

Promoter

CMV

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cyto-CaNAR2

Plasmid

#64729

Purpose

Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al Elife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2-NES

Use

Tags

Cerulean3, Nuclear export signal (NES), and YPet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-IQGAP1

Plasmid

#30118

Depositor

David Sacks

Article

Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

Insert

IQGAP1 (IQGAP1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA-D1cpV

Plasmid

#37479

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1cpV second generation cameleon (calcium sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
Lyn-R-GECO1

Plasmid

#120410

Purpose

Expresses a fusion between myristoylation signal peptide and R-GECO1. This construct targets R-GECO1 to the plasma membrane.

Depositor

Won Do Heo

Article

Kim et al Proc Natl Acad Sci U S A. 2016 May 24;11

Insert

Lyn-R-GECO1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-Mito4x-jRCaMP1b

Plasmid

#127873

Purpose

Red-shifted fluorescent reporter for mitochondrial matrix Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

Red-shifted mitochondrial matrix Ca2+ indicator

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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TPC1-G-GECO1.2

Plasmid

#207143

Purpose

Fusion of Ca2+ reporter and endosomal Ca2+-permeable channel

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

TPCN1 (TPCN1 Human)

Use

Tags

G-GECO1.2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-OMM-jRCaMP1b

Plasmid

#127871

Purpose

Red-shifted fluorescent reporter for mitochondrial outer membrane Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

OMM-jRCaMP1b (Red-shifted outer membrane Ca2+ indicator)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTH1-SpSrtA

Plasmid

#64987

Purpose

Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

S. pyogenes sortase A

Use

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-81

Promoter

T7 promoter

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Cyto-CaNARi

Plasmid

#64849

Purpose

Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

Depositor

Jin Zhang

Article

Mehta et al Elife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNARi

Use

Tags

Cerulean, Nuclear export signal (NES), and Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-PM-CaNAR2

Plasmid

#64730

Purpose

Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al Elife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

Lyn-CaNAR2

Use

Tags

Cerulean3, N-terminal targeting sequence from Lyn…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-lynD3cpV

Plasmid

#37472

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D3cpv second generation cameleon (calcium sensor)

Use

Tags

the myristoylation and palmitoyla- tion sequence …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-PM-CaNARi

Plasmid

#64850

Purpose

Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

Depositor

Jin Zhang

Article

Mehta et al Elife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

Lyn-CaNARi

Use

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-D1

Plasmid

#37480

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1 second generation cameleon (calcium sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
CaYin1 NES

Plasmid

#33318

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

CaYin1 NES

Use

Tags

NES sequence LALKLAGLDIGS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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CaYin1

Plasmid

#33067

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

CaYin1

Use

Tags

6 His tag

Expression

Bacterial

Mutation

Promoter

ARA promoter

Available since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-KA

Plasmid

#64981

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Use

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Promoter

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge