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Plasmid#148000PurposeMammalian Expression of HsNot1iso1_840-1605-del1097-1110-RNFtoAAADepositorInsertHsNot1iso1_840-1605-del1097-1110-RNFtoAAA (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_DED/AAA 535-888 USP7
Plasmid#131255PurposeMammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationA mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AAA-nLuc-3XFLAG-CL1/PEST
Plasmid#127320PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AAA start codon (Negative control plasmid)DepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-FIT2-FLL[157-159]AAA
Plasmid#96994PurposeExpress GFP-tagged mutated mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsGFPExpressionPlantMutationAmino acid residues 157-159 were mutated (FLL[157…Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC32-FIT2-FLL[157-159]AAA
Plasmid#96991PurposeExpress mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsExpressionPlantMutationAmino acid residues 157-159 were mutated (FLL[157…Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
(424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A)
Plasmid#168191PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to an inactive truncated OGT of 4 TPRs with K852A mutationDepositorInsertHA-nLag6-(EAAAK)4-OGT(4,K852A)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
(344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)
Plasmid#168097PurposeHA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.DepositorInsertHA-nGFP-(EAAAK)4-OGA(544-706)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
(271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706)
Plasmid#168102PurposeHA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGADepositorInsertHA-nEPEA-(EAAAK)3-OGA(544-706)
UseTagsHA-tagExpressionMammalianMutationOGA truncated to contain residues 544-706PromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
(367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706)
Plasmid#168099PurposeHA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGADepositorInsertHA-nUbc-OGA(544-706)
UseTagsHA-tagExpressionMammalianMutationOGA truncated to contain residues 544-706PromoterT7/CMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
(369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706)
Plasmid#168190PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGA with residues 544-706. For use with myc-OGA(1-400) split-OGA system.DepositorInsertHA-nLag6-(EAAAK)4-OGA(544-706)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 N481K (AAA)
Plasmid#212562PurposeEncodes SARS-CoV-2 spike N481K for pseudovirus productionDepositorInsertSARS-CoV-2 Spike N481K (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationN481KPromoterCAGAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 E554K (AAA)
Plasmid#212566PurposeEncodes SARS-CoV-2 spike E554K for pseudovirus productionDepositorInsertSARS-CoV-2 Spike E554K (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationE554KPromoterCAGAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 N460K (AAA)
Plasmid#212561PurposeEncodes SARS-CoV-2 spike N460K for pseudovirus productionDepositorInsertSARS-CoV-2 Spike N460K (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationN460KPromoterCAGAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 T547K (AAA)
Plasmid#212478PurposeEncodes SARS-CoV-2 spike T547K for pseudovirus productionDepositorInsertSARS-CoV-2 Spike T547K (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationT547KPromoterCAGAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 N679K (AAA)
Plasmid#212479PurposeEncodes SARS-CoV-2 spike N679K for pseudovirus productionDepositorInsertSARS-CoV-2 Spike N679K (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationN679KPromoterCAGAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 N764K (AAA)
Plasmid#212481PurposeEncodes SARS-CoV-2 spike N764K for pseudovirus productionDepositorInsertSARS-CoV-2 Spike N764K (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationN764KPromoterCAGAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 R346K (AAA)
Plasmid#212451PurposeEncodes SARS-CoV-2 spike R346K for pseudovirus productionDepositorInsertSARS-CoV-2 Spike R346K (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationR346KPromoterCAGAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 E154K (AAA)
Plasmid#212420PurposeEncodes SARS-CoV-2 spike E154K for pseudovirus productionDepositorInsertSARS-CoV-2 Spike E154K (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationE154KPromoterCAGAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 T478K (AAA)
Plasmid#212422PurposeEncodes SARS-CoV-2 spike T478K for pseudovirus productionDepositorInsertSARS-CoV-2 Spike T478K (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationT478KPromoterCAGAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 E484K (AAA)
Plasmid#212348PurposeEncodes SARS-CoV-2 spike E484K for pseudovirus productionDepositorInsertSARS-CoV-2 Spike E484K (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationE484KPromoterCAGAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only