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Showing: 201 - 220 of 2919 results

  2. pcDNA3.1-N-Myc_DED/AAA 535-888 USP7

    Plasmid
    #131255
    Purpose
    Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    A mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    131255-plasmid-map-sequence-id-267709

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  4. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

    Plasmid
    #202571
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Use
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    202571-plasmid-map-sequence-id-429226

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  16. pT7-EGFP-C1-HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres_W

    Plasmid
    #147887
    Purpose
    Mammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres
    Depositor
    Elisa Izaurralde
    Article
    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:
    Insert
    HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres (CNOT1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    1 silent mutation compared to the sequence of iso…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    147887-plasmid-map-sequence-id-290334

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  19. pcDNA-rUSP19ER-siRNAresistant

    Plasmid
    #155251
    Purpose
    for mammalian overexpression of the siRNA resistant ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) siRNA resistant (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    155251-plasmid-map-sequence-id-313953

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  20. pcDNA-rUSP19CYT-siRNAresistant

    Plasmid
    #155252
    Purpose
    for mammalian overexpression of the siRNA resistant CYT isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (CYT isoform) siRNA resistant (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    155252-plasmid-map-sequence-id-313955

    Enlarge

Showing: 201 - 220 of 2919 results