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G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_noTerm_attP(GA)_IRES-UnaG-2A-Puro-Term

Plasmid

#200634

Purpose

Plasmid self-excising recombination plasmid for Matreyek Bxb1(GT) landing pad

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

PTEN (PTEN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

Plasmid

#68357

Purpose

The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.

Depositor

Jannik Elverløv-Jakobsen

Inserts

5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
FlpO recombinase

Use

CRISPR

Tags

mTurquoise2 (BFP)

Expression

Mammalian

Mutation

Promoter

U6 and synthetic Probasin ARRx2

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2344

Plasmid

#36764

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PTEN-TALEN_Left (Pten Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2345

Plasmid

#36765

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PTEN-TALEN_Right (Pten Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pGL3-Promoter-hPE(+)-Luc

Plasmid

#172003

Purpose

Luciferase Reporter for Human PTEN Enhancer PE - Forward Orientation

Depositor

Daniel Herranz

Article

Tottone et al Blood Cancer Discov. 2021 Jan;2(1):92-10

Insert

Human PE sequence (hPE)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

SV40 Promoter

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Promoter-hPE(-)-Luc

Plasmid

#172004

Purpose

Luciferase Reporter for Human PTEN Enhancer PE - Reverse Orientation

Depositor

Daniel Herranz

Article

Tottone et al Blood Cancer Discov. 2021 Jan;2(1):92-10

Insert

Human PE sequence (hPE)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

SV40 Promoter

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Promoter-mPE(-)-Luc

Plasmid

#172006

Purpose

Luciferase Reporter for Mouse PTEN Enhancer PE - Reverse Orientation

Depositor

Daniel Herranz

Article

Tottone et al Blood Cancer Discov. 2021 Jan;2(1):92-10

Insert

Mouse PE sequence (mPE)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

SV40 Promoter

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Promoter-mPE(+)-Luc

Plasmid

#172005

Purpose

Luciferase Reporter for Mouse PTEN Enhancer PE - Forward Orientation

Depositor

Daniel Herranz

Article

Tottone et al Blood Cancer Discov. 2021 Jan;2(1):92-10

Insert

Mouse PE sequence (mPE)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

SV40 Promoter

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIPZ

Plasmid

#206981

Purpose

empty pTRIPZ vector for cloning of doxycycline-inducible gene expression

Depositor

Ming Chen

Article

Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FUG-T2A-cre

Plasmid

#66691

Purpose

Recombine floxed alleles in any cell

Depositor

Bryan Luikart

Article

Williams et al J Neurosci. 2015 Jan 21;35(3):943-59. do

Insert

GFP-T2A-Cre

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only
Fuc-T2A-cre

Plasmid

#66690

Purpose

Recombine floxed alleles in any cell

Depositor

Bryan Luikart

Article

Williams et al J Neurosci. 2015 Jan 21;35(3):943-59. do

Insert

mCherry-T2A-Cre

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only
pRubiG-T2A-cre

Plasmid

#66693

Purpose

Recombine floxed alleles in newborn neurons

Depositor

Bryan Luikart

Article

Williams et al J Neurosci. 2015 Jan 21;35(3):943-59. do

Insert

GFP-T2A-cre

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only
pRubiC-T2A-cre

Plasmid

#66692

Purpose

Recombine floxed alleles in newborn neurons

Depositor

Bryan Luikart

Article

Williams et al J Neurosci. 2015 Jan 21;35(3):943-59. do

Insert

mCherry-T2A-Cre

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3Basic-Mdm2-T3

Plasmid

#32369

Depositor

Hong Wu

Article

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pGL3Basic-Mdm2-T4

Plasmid

#32370

Depositor

Hong Wu

Article

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pPI356

Plasmid

#207449

Purpose

Extrachromosomal expression vector PkgE-PH-GFP and PTEN-mCherry

Depositor

Rob Kay

Article

Lutton et al Curr Biol. 2023 Aug 7;33(15):3083-3096.e

Inserts

PkgE
PTEN

Use

Unspecified

Tags

GFP and mCherry

Expression

Mutation

PH domain only

Promoter

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3Basic-Mdm2-T2

Plasmid

#32366

Depositor

Hong Wu

Article

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
PGL3Basic-Mdm2-T1

Plasmid

#32365

Depositor

Hong Wu

Article

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pGL3Basic-Mdm-P1

Plasmid

#32367

Depositor

Hong Wu

Article

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pGL3Basic-Mdm3-T5

Plasmid

#32373

Depositor

Hong Wu

Article

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only