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PRKAR2A gRNA (BRDN0001145818)

Plasmid

#77672

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR2A

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR2A (PRKAR2A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKAR2A gRNA (BRDN0001145599)

Plasmid

#77673

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR2A

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR2A (PRKAR2A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKAR2B gRNA (BRDN0001147301)

Plasmid

#76150

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR2B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR2B (PRKAR2B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKAR2B gRNA (BRDN0001147315)

Plasmid

#76151

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR2B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR2B (PRKAR2B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKAR2B gRNA (BRDN0001147707)

Plasmid

#76152

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR2B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR2B (PRKAR2B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLAP-MIS12-KARD

Plasmid

#114057

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD (BUB1B Human)

Use

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Nuc-deltaCKAR

Plasmid

#222430

Purpose

FRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Nucleus delta-selective C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Mito(OM)-deltaCKAR

Plasmid

#222431

Purpose

FRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Mitochondrial outer membrane delta-selective C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Golgi-deltaCKAR

Plasmid

#222428

Purpose

FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Golgi delta-selective C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FluoSTEP-AKAR (C/Y)

Plasmid

#181862

Purpose

FluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-AKAR (C-Y)

Use

Tags

CFP(1-10) and cpVenus(E127)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-Fluc-oskar_C

Plasmid

#146022

Purpose

Insect Expression of oskar-3UTR

Depositor

Elisa Izaurralde

Insert

oskar-3UTR (osk Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmOskar_C

Plasmid

#146008

Purpose

Insect Expression of DmOskar

Depositor

Elisa Izaurralde

Insert

DmOskar (osk Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_KARS_tRNA-anti-codon

Plasmid

#109865

Purpose

Protein expression and purification of KARS_tRNA-anti-codon

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

KARS_tRNA-anti-codon (KARS1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLAP-MIS12-KARD-3D

Plasmid

#114058

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD-3D (BUB1B Human)

Use

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

BUBR1-S670D, S676D and T680D

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLAP-MIS12-KARD-3A

Plasmid

#114059

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD-3A (BUB1B Human)

Use

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

BUBR1-S670A, S676A and T680A

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKAR1A gRNA (BRDN0001144984)

Plasmid

#77721

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR1A

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR1A (PRKAR1A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKAR1A gRNA (BRDN0001146809)

Plasmid

#77722

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR1A

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR1A (PRKAR1A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKAR1B gRNA (BRDN0001146606)

Plasmid

#76838

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR1B (PRKAR1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKAR1B gRNA (BRDN0001144765)

Plasmid

#76839

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR1B (PRKAR1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKAR1B gRNA (BRDN0001147089)

Plasmid

#76840

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR1B (PRKAR1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge