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pAG1171 FIREmate-ECFP-Giantin

Plasmid

#205166

Purpose

Expresses FIREmate-ECFP-Giantin in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

FIREmate-ECFP-Giantin

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Lck-FRB-ECFP(W66A)

Plasmid

#67902

Purpose

Expresses a membrane located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van_Unen et al Scientific Reports 5, Article number: 14

Insert

Lck-FRB-ECFP(W66A)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yECFP-Hygro

Plasmid

#44647

Depositor

Kurt Thorn

Insert

yEmCFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pAG1243 NES-ECFP-FIREmate

Plasmid

#205169

Purpose

Expresses NES-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

NES-ECFP-FIREmate

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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FRB-ECFP(W66A)-Giantin

Plasmid

#67903

Purpose

Expresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van_Unen et al Scientific Reports 5, Article number: 14

Insert

FRB-ECFP(W66A)-Giantin

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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KN206: pMVP/eCFP-DEST

Plasmid

#121854

Purpose

pMVP destination vector, empty expression vector backbone w/ eCFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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optGluCl beta Y182F ECFP

Plasmid

#15105

Depositor

Henry Lester

Article

Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

Insert

GluCl beta (glc-2 Nematode)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Codon optimized cDNA, Y182F

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

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P4U2C8T2-v1-1 (Pl-tetO1-deCFP) (135p)

Plasmid

#70211

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deCFP

Use

Tags

Expression

Mutation

Promoter

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLCMV:ECFP(loxP)(FRT)HNF1B

Plasmid

#31439

Depositor

Gerhart Ryffel

Article

Sauert et al PLoS One. 2012;7(3):e33522. Epub 2012 Ma

Insert

HNF1B (HNF1B Human)

Use

Cre/Lox; Flp/frt

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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LCMV:ECFP(loxP)(FRT)MCS

Plasmid

#31377

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

ECFP(loxP)MCS

Use

Cre/Lox; Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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KN302: pMVP/SB/eCFP-DEST

Plasmid

#121863

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eCFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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V956 pLPS-3'ECFP - Acceptor

Plasmid

#11698

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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LB801: pMVP/PB/eCFP-DEST

Plasmid

#121872

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ eCFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLCMV:ECFP(loxP)(FRT)HNF1B_P328L329del

Plasmid

#30528

Depositor

Gerhart Ryffel

Article

Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

Insert

HNF1B (HNF1B Human)

Use

Cre/Lox; Flp/frt

Tags

Myc

Expression

Mammalian

Mutation

P328L329delCCTCT

Promoter

Available since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW eCFP-T2A-mCherry

Plasmid

#118421

Purpose

Produces equimolar expression of eCFP and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

eCFP-T2A-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-R4r-ECFP-R3r

Plasmid

#32594

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

ECFP

Use

Gateway cloning vector

Tags

Expression

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pLCMV:ECFP(loxP)(FRT)HNF1B_A263ins

Plasmid

#31440

Depositor

Gerhart Ryffel

Article

Sauert et al PLoS One. 2012;7(3):e33522. Epub 2012 Ma

Insert

HNF1B (HNF1B Human)

Use

Cre/Lox; Flp/frt

Tags

Myc

Expression

Mammalian

Mutation

A263insGG

Promoter

Available since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TOgw-C-mECFP

Plasmid

#223950

Purpose

Gateway cloning of genes of interest with a C-terminal mECFP fusion; generation of stable cells with doxycycline inducible expression

Depositor

Karla Feijs, Roko Žaja

Type

Empty backbone

Use

Tags

mECFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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Slack Slo2.2 max1-ECFP

Plasmid

#197318

Purpose

Plasmid expressing an antigen used to generate an antibody that targets the Slack K+ channel subunit

Depositor

Christopher Lingle

Insert

KCNT1/Slo2.2/Slack K+ channel (Kcnt1 Mouse)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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LD101: pMVP (L3-L2) eCFP + WPRE

Plasmid

#121775

Purpose

pMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP + WPRE

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge