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GFP-BioID-FAM21
Plasmid#121046PurposeExpresses GFP tagged BioID and human FAM21C in mammalian cellsDepositorInsertsUseTagsGFPExpressionMammalianMutationR118G (highly promiscuous form)PromoterCMVAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2
Plasmid#196250PurposePlasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119DepositorInsertattL1-GlpT-scGFP-attL2
UseTagsExpressionBacterialMutationPromoterJ23119 promoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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SiRES COR1C-GFP
Plasmid#195154Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
UseTagsExpressionMammalianMutationmutations to make si RNA resistantPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
SDHC-pHLuorin-sems
Plasmid#107767Purposelabeling of mitochondrial succinate dehydrogenase subunit C, pH measurementsDepositorInsertSubunit C of SDH fused to pHluorin (SDHC Human)
UseTagspHluorinExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
SiRES COR1C DCC-GFP
Plasmid#195149Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
UseTagsExpressionMammalianMutationmutations to make si RNA resistant and remove coi…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
SiRES COR1C ACT--GFP
Plasmid#195151Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
UseTagsExpressionMammalianMutationmutations to make si RNA resistant and remove act…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NPC1(WT)-FLAG
Plasmid#164972PurposeLentiviral vector for NPC1 expression in mammalian cellsDepositorInsertNPC1 (NPC1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
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SNAP-Sec61B
Plasmid#141152PurposeFluorescent labeling of ER membrane in mammalian cellsDepositorInsertSec61 beta (SEC61B Human)
UseTagsSNAP tagExpressionMammalianMutationL79M on Sec61BPromoterCMVAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NPC1(P691S)-FLAG
Plasmid#164973PurposeLentiviral vector for NPC1 expression in mammalian cellsDepositorInsertNPC1 (NPC1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationP691SPromoterEF1aAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NPC1(F503/4A)-FLAG
Plasmid#164974PurposeLentiviral vector for NPC1 expression in mammalian cellsDepositorInsertNPC1 (NPC1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationF503A and F504APromoterEF1aAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GA-MFF
Plasmid#141160PurposeDimerization-dependent green fluorescent protein targeted to mitochondrial outer membraneDepositorInsertmitochondrial fission factor (MFF Human)
UseTagsGAExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
B-MFF
Plasmid#141158PurposeDimerization-dependent fluorescent protein binding partner targeted to outer mitochondrial membraneDepositorInsertmitochondrial fission factor (MFF Human)
UseTagsGBExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTet-On-TAP
Plasmid#59017PurposeConstruct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.DepositorTypeEmpty backboneUseToxoplasma expressionTagsCalmodulin Binding Peptide fused with a TEV Cleav…ExpressionMutationPromoterRPS13 with Tet Operators InsertedAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
mMaple-OMP25
Plasmid#141151PurposePhotoconvertible fluorescent protein targeted to the outer mitochondrial membrane (OMM) in mammalian cells.DepositorInsertOMP25 (Synj2bp Rat)
UseTagsmMapleExpressionMammalianMutationOMP25 c terminus from addgene plasmid #69598PromoterCMVAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NPC1(P202A/F203A)-FLAG
Plasmid#164976PurposeLentiviral vector for NPC1 expression in mammalian cellsDepositorInsertNPC1 (NPC1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationP202A/ F203APromoterEF1aAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
SNAP-OMP25
Plasmid#141149PurposeFluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cellsDepositorInsertOMP25 (Synj2bp Rat, Budding Yeast)
UseTagsSNAP tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro GFP-REX1-IRES-mCherry
Plasmid#232239PurposeMammalian expression of REX1-GFP-IRES-mCherryDepositorInsertREX1-GFP-IRES-mCherry (Zfp42 Mouse)
UseTagsAcGFP1ExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only
