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Plasmid#55311PurposeLocalization: Microtubules, Excitation: 399, Emission: 456DepositorInsertMAPTau (MAPT Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP2-Fibrillarin-7
Plasmid#55241PurposeLocalization: Nucleoli, Excitation: 383, Emission: 448DepositorInsertFibrillarin (FBL Human)
UseTagsEBFP2ExpressionMammalianMutationR36Q, S66FPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTS2337_Tier3(SB)-TagBFP2_Puro
Plasmid#169635PurposeTier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mTagBFP2 and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Dectin1A-C-10
Plasmid#55290PurposeLocalization: Membrane, Excitation: 399, Emission: 456DepositorInsertDectin1A (CLEC7A Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagBFP2-Caveolin-C-10
Plasmid#55279PurposeLocalization: Membrane, Excitation: 399, Emission: 456DepositorInsertCaveolin (CAV1 Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagBFP2-CAF1-C-10
Plasmid#55277PurposeLocalization: Chromatin Assembly Factor 1, Excitation: 399, Emission: 456DepositorInsertCAF1
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagBFP2-MAPTau-N-10
Plasmid#55312PurposeLocalization: Microtubules, Excitation: 399, Emission: 456DepositorInsertMAPTau (MAPT Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagBFP2-Vinculin-23
Plasmid#55334PurposeLocalization: Focal Adhesions, Excitation: 399, Emission: 456DepositorInsertVinculin (VCL Human)
UseTagsmTagBFP2ExpressionMammalianMutationV234L in Vinculin.PromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pME-TagBFP-NS
Plasmid#75342PurposeMultisite gateway entry clone for adding N-terminal fusions of TagBFPDepositorInsertTagBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Nucleus-7
Plasmid#55249PurposeLocalization: Nuclear Localization, Excitation: 383, Emission: 448DepositorInsertSV40 T-Antigen Nuclear Localization Signal (NLS)
UseTagsEBFP2-NLSExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagBFP2-MCAK-C-7
Plasmid#62785PurposeLocalization: Microtubules/Kinesin, C1 Cloning Vector, Excitation:399, Emission:456DepositorInsertMCAK (KIF2C Human)
UseTagsmTagBFP2ExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
ptagBFP-LacI
Plasmid#103839PurposeFlourescent marker for lacO arraysDepositorInserttagBFP-LacI
UseTagsExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterSV2Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Annexin-12
Plasmid#55276DepositorInsertAnnexin (A4) (ANXA4 Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYES-mtBFP
Plasmid#45055DepositorInsertmtBFP
UseTagsExpressionYeastMutationPromoterGALAvailable sinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-mTagBFP2
Plasmid#184940PurposeER Visualization (OSER assay) Expresses mTagBFP2 in fusion with N-terminal part of p450DepositorInsertCytERM-mTagBFP2
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-PIP4K2C
Plasmid#202730Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:PIP4K2C (PIP4K2C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_AP4-PDGFR_EF1a_eBFP2
Plasmid#186306PurposePlasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.DepositorInsertsAP4-PDGFR
eBFP2
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-CENPB-N-22
Plasmid#55237PurposeLocalization: Nucleus/Centromeres, Excitation: 383, Emission: 448DepositorInsertCENPB (CENPB Human)
UseTagsEBFP2ExpressionMammalianMutationaa 1-169 of CENPB (DNA Binding Domain)PromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNLS-2xBFP(4C)
Plasmid#176151PurposeEncodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.DepositorInsertNLS-2xBFP(4C)
UseTags6xHis tag and SV40 nuclear localization sequence …ExpressionBacterialMutationCompared with EGFP, the two BFP domains have the …PromoterPtrcAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-TagBFP2-NS
Plasmid#80502PurposeMultisite gateway entry clone for adding N-terminal fusions of TagBFP2DepositorInsertTagBFP2 (no STOP)
UseMultisite gateway middle entry cloneTagsExpressionMutationPromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only