Addgene

We narrowed to 751 results for: Anti-CRISPR

Showing: 201 - 220 of 751 results

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pMpGE_En03

Plasmid

#71535

Purpose

Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi, Sumihare Noji

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGE011

Plasmid

#71537

Purpose

For CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGE_En01

Plasmid

#71534

Purpose

Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Ikuko Hara-Nishimura, Takayuki Kohchi

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pWT-IFl_WT-IFr

Plasmid

#192207

Purpose

Wildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and wildtype I-F Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pScr-IFl_WT-IFr

Plasmid

#192208

Purpose

Scrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Scrambled I-F Leader and wildtype I-F Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAcrF2

Plasmid

#89234

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage site

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Insert

AcrF2

Use

Tags

6xHis-TEV

Expression

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAcrF1

Plasmid

#89233

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF1 (gene 35 from bacteriophage JBD30), with N-terminal 6his tag and TEV protease cleavage site

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Insert

AcrF1

Use

Tags

6xHis-TEV and FLAG

Expression

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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phU6 ST

Plasmid

#188712

Purpose

sgRNA plasmid encoding hU6 promoter driving an anti-safe targeting sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

Safe Targeting sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pmU6 ST

Plasmid

#188713

Purpose

sgRNA plasmid encoding mU6 promoter driving an anti-safe targeting sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

Safe Targeting sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p7SK ST

Plasmid

#188714

Purpose

sgRNA plasmid encoding 7SK promoter driving an anti-safe targeting sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

Safe Targeting sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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phH1 ST

Plasmid

#188715

Purpose

sgRNA plasmid encoding hH1 promoter driving an anti-safe targeting sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

Safe Targeting sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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GST-beta catenin tag

Plasmid

#186422

Purpose

Encodes GST fused to beta catenin tag recognized by anti-beta catenin nanobody

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

GST-beta catenin tag

Use

Tags

6His and GST-beta catenin tag (PDRKAAVSHWQQRS)

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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phU6 NFATC2

Plasmid

#188708

Purpose

sgRNA plasmid encoding hU6 promoter driving an anti-NFATC2 sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pmU6 NFATC2

Plasmid

#188709

Purpose

sgRNA plasmid encoding mU6 promoter driving an anti-NFATC2 sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p7SK NFATC2

Plasmid

#188710

Purpose

sgRNA plasmid encoding 7SK promoter driving an anti-NFATC2 sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
phH1 NFATC2

Plasmid

#188711

Purpose

sgRNA plasmid encoding hH1 promoter driving an anti-NFATC2 sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCI152-pLenti-SiT-Cas12a

Plasmid

#128405

Purpose

Express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAGHA-SCAF8

Plasmid

#122470

Purpose

Dox-inducible FLAG/HA (N-terminal tag) SCAF8 expression

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF8 isoform C CRISPR resistant (SCAF8 Human)

Use

Tags

FLAGHA

Expression

Mammalian

Mutation

Synonymous mutations conferring resistance to gRN…

Promoter

CMV

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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P4 cosmid

Plasmid

#196335

Purpose

P4 cosmid with CRISPR-Cas9 system

Depositor

Baojun Wang

Article

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Insert

genes from phage P4, CRISPR-Cas9 system

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCE048-SiT-Cas12a

Plasmid

#128124

Purpose

Express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge