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pcDNA3.1 GIL-11 myc TEV TwinStrep

Plasmid

#199627

Purpose

Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

Depositor

Jurgen Scheller

Article

Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

Insert

GIL-11

Use

Tags

TwinStrep-tag

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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ATF4 11: 5’ATF4.uORF2.GFP

Plasmid

#21858

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

upstream ORF2 (uORF) of ATF4 fused to EGFP

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 HA-TSPAN12^11-LEL

Plasmid

#115785

Purpose

Expresses a chimera in mammalian cells: the large extracellular loop of TSPAN12 is replaced with TSPAN11 sequence

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

TSPAN12/11 chimera (TSPAN11, TSPAN12 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

large extracellular loop of TSPAN12 replaced with…

Promoter

CMV

Available since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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1203N-CMV-dDiCas7-11-NLS-bGHpolyA

Plasmid

#221537

Purpose

Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain

Depositor

Omar Akbari

Insert

DiCas7-11

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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1203O-CMV-DiCas7-11-Δ7.1-bGHpolyA

Plasmid

#220959

Purpose

DiCas7-11 effector with Cas7.1 domain deletion mutation

Depositor

Omar Akbari

Insert

DiCas7-11

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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L2379-FKBP-GPA(V84R)-20-11S

Plasmid

#222901

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA(V84R)-20GS-NanoLuc11S

Use

Tags

Myc

Expression

Mammalian

Mutation

V84R in GPA

Promoter

CMV

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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L2378-FRB-GPA(V84R)-20-11S

Plasmid

#222899

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA(V84R)-20GS-NanoLuc11S

Use

Tags

Myc

Expression

Mammalian

Mutation

V84R in GPA

Promoter

CMV

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ETS1(11))-PGKpuro2ABFP-W

Plasmid

#208549

Purpose

Lentiviral vector expressing gRNA targeting human ETS1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ETS1(11) (ETS1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(TAL1(11))-PGKpuro2ABFP-W

Plasmid

#208545

Purpose

Lentiviral vector expressing gRNA targeting human TAL1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

TAL1(11) (TAL1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RUNX1(11))-PGKpuro2ABFP-W

Plasmid

#200500

Purpose

Lentiviral vector expressing gRNA targeting human RUNX1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RUNX1(11) (RUNX1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(NDUFA1(11))-PGKpuro2ABFP-W

Plasmid

#200494

Purpose

Lentiviral vector expressing gRNA targeting human NDUFA1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

NDUFA1(11) (NDUFA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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Kv2.1 K+ channel scFv [L83/11]

Plasmid

#190519

Purpose

Mammalian Expression of Kv2.1 K+ channel scFV. Derived from hybridoma L83/11.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv2.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcnb1 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Kir2.4 K+ channel [N465/11]

Plasmid

#190332

Purpose

Mammalian Expression Plasmid of anti-Kir2.4 K+ channel (Mouse). Derived from hybridoma N465/11.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kir2.4 K+ channel (Mus musculus) recombinant Mouse monoclonal antibody (Kcnj14 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO SnRK2.6 (11-362)

Plasmid

#177847

Purpose

Bacterial Expression of SnRK2.6

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2.6 (OST1 Mustard Weed)

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pattB-synaptobrevin-11-QFMD262-475-hsp70

Plasmid

#46120

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QFMD262-475

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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GFP1-10-miniCMV-GFP11×11-GFP-tDeg

Plasmid

#185404

Purpose

Expresses GFP1-10 and GFP11×11-GFP-tDeg

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

GFP1-10

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR

Plasmid

#200637

Purpose

Recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

Vif

Use

Tags

Expression

Mammalian

Mutation

T27stop

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDF0387 pcmv-hudiscas7-11-r978-gggs-r1294

Plasmid

#186984

Purpose

mammalian expression of cas7-11-r978-gggs-r1294

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-r978-gggs-r1294

Use

Tags

Expression

Mammalian

Mutation

r978-gggs-r1294 deletion

Promoter

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDF0237 pCMV - dead huDisCas7-11 - NES - huADAR2

Plasmid

#172510

Purpose

Encodes human codon optimized catalytically dead DisCas7-11-NES-huADAR2 fusion protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - dead huDisCas7-11 - NES - huADAR2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0386 pcmv-hudiscas7-11-s1006-gggs-d1221

Plasmid

#186983

Purpose

mammalian expression of cas7-11-s1006-gggs-d1221

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-s1006-gggs-d122

Use

Tags

Expression

Mammalian

Mutation

s1006-gggs-d1221 deletion

Promoter

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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