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TU#1805_CRISPR_unc-73_exon2

Plasmid

#82359

Purpose

to create unc-73B null allele

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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CTHRC1A-c001

Plasmid

#205105

Purpose

CTHRC1 protein expression

Depositor

Paul Brennan

Insert

CTHRC1 (CTHRC1 Human)

Use

Tags

AviTag-6His and PTPRSsp

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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CAPN2A-c002

Plasmid

#205104

Purpose

CAPN2 protein expression

Depositor

Paul Brennan

Insert

CAPN2 (CAPN2 Human)

Use

Tags

6His-TEV and AviTag

Expression

Bacterial

Mutation

WT

Promoter

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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GPC5A-c001

Plasmid

#205110

Purpose

GPC5 protein expression

Depositor

Paul Brennan

Insert

GPC5 (GPC5 Human)

Use

Tags

AviTag-6His and PTPRSsp

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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MDKA-c012

Plasmid

#205113

Purpose

MDK protein expression

Depositor

Paul Brennan

Insert

MDK (MDK Human)

Use

Tags

AviTag-6His and PTPRSsp

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCSFLPe

Plasmid

#31130

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

FLP recombinase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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cp193g7

Plasmid

#25950

Depositor

Robert Campbell

Article

Carlson et al Protein Sci. 2010 Jun 2. ():.

Insert

cp193g7

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only
pKT0176

Plasmid

#8743

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

tdimer2

Use

Tags

Codon optimized linker

Expression

Yeast

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pKT0178

Plasmid

#8744

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

tdimer2

Use

Tags

Codon optimized linker

Expression

Yeast

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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cp193g7N

Plasmid

#25982

Depositor

Robert Campbell

Article

Carlson et al Protein Sci. 2010 Jun 2. ():.

Insert

cp193g7N

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2010

Availability

Academic Institutions and Nonprofits only
pKT0146

Plasmid

#8742

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

tdimer2

Use

Tags

Codon optimized linker

Expression

Yeast

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pTY1048-S65T-LacI

Plasmid

#34853

Depositor

Paul Sternberg

Article

Gonzalez-Serricchio et al BMC Genet. 2006 Jun 7;7:36.

Insert

GFP-LacI

Use

Tags

Expression

Worm

Mutation

GFP is mutated: S65T and contains introns, in ord…

Promoter

Available since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only
RS314

Plasmid

#17542

Depositor

Bert Vogelstein

Article

Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
cp204

Plasmid

#25951

Depositor

Robert Campbell

Article

Carlson et al Protein Sci. 2010 Jun 2. ():.

Insert

cp204

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only
M71 TV

Plasmid

#15635

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

M71 Targeting Vector

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
cp196

Plasmid

#25952

Depositor

Robert Campbell

Article

Carlson et al Protein Sci. 2010 Jun 2. ():.

Insert

cp196

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only
pD2eGFP-5xUTR

Plasmid

#31121

Depositor

Christopher Sullivan

Article

Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

Insert

5xoligonucleotides eGFPutr

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pUK21-deltaBB

Plasmid

#51658

Purpose

Bacterial cloning vector based on pUK21 (Kan)

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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cp201

Plasmid

#25953

Depositor

Robert Campbell

Article

Carlson et al Protein Sci. 2010 Jun 2. ():.

Insert

cp201

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only
P2 TV

Plasmid

#15631

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

P2 Targeting Vector

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
P3 TV

Plasmid

#15632

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

P3 Targeting Vector

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
M50 TV

Plasmid

#15633

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

M50 Targeting Vector

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
P4 TV

Plasmid

#15634

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

P4 Targeting Vector

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
GPNMBA-c200

Plasmid

#205111

Purpose

GPNMB protein expression

Depositor

Paul Brennan

Insert

GPNMB (GPNMB Human)

Use

Tags

AviTag-6His and PTPRSsp

Expression

Mammalian

Mutation

WT

Promoter

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBA310

Plasmid

#190002

Purpose

Inducible expression vector for Trypanosoma brucei

Depositor

Bungo Akiyoshi

Article

Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do

Type

Empty backbone

Use

Expression vector for trypanosoma brucei

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pUC21-deltaBB

Plasmid

#51657

Purpose

Bacterial cloning vector based on pUC21 (Amp)

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-neo-CD2-FAK

Plasmid

#37013

Depositor

Bob Weinberg

Article

Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

Insert

CD2-FAK (CD2, PTK2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only

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HA-14-3-3 zeta

Plasmid

#116888

Purpose

Expresses HA-tagged human 14-3-3 zeta in mammalian cells

Depositor

Ken-Ichi Takemaru, FENG-QIAN Li

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 zeta (YWHAZ Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Lmx1b

Plasmid

#34997

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

LIM homeobox transcription factor 1-beta (Lmx1b Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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L_NLS 41kDa

Plasmid

#201342

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A4-EGFP-2PrA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pIA1385

Plasmid

#166862

Purpose

Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pENO1-BFP-NAT

Plasmid

#50046

Purpose

Expression of BFP in Candida albicans

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

BFP

Use

Tags

Expression

Yeast

Mutation

Promoter

pENO1

Available since

Jan. 26, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.18 CMV-Luc

Plasmid

#100984

Purpose

CMV luciferase reporter - firefly luciferase driven by CMV promoter

Depositor

Lee Helman

Article

Grohar et al J Natl Cancer Inst. 2011 Jun 22;103(12):

Insert

CMV

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-mCherry-Vav1

Plasmid

#50044

Purpose

Expression of mCherry-Vav1 in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

Vav1 (Vav1 Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
3xFlag-eGFP-Flag-SETX

Plasmid

#218838

Purpose

Mammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.

Depositor

Albert La Spada

Article

Bennett et al Heliyon. 2020 Jun 13;6(6):e04165. doi: 1

Insert

Senataxin (SETX Human)

Use

Tags

Flag and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PR_GEF YFP (2XPDZ-YFP-LARG DH)

Plasmid

#80408

Purpose

Recruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

Depositor

Michael Glotzer

Article

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

2XPDZ_YFP_LARG DH (aa 766-997) (ARHGEF12 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

Plasmid

#119074

Purpose

Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

ubb:MCP-p65-HSF1-2a-Cerulean

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish ubiquitin promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

Plasmid

#119075

Purpose

Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

ubb:Com-KRAB-2a-Cerulean

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish ubiquitin promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC-Ds-DrU6a:sgRNA-com-Ds

Plasmid

#119077

Purpose

To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:2xCom-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC-Ds-DrU6a:sgRNA-MS2-Ds

Plasmid

#119076

Purpose

To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:2xMS2-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-myc-PDGFRbeta

Plasmid

#136455

Purpose

Mammalian expression plasmid for c-myc-tagged PDGFRB

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

PDGFRbeta (PDGFRB Human)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1alpha-EPO-218-PDGFR

Plasmid

#139057

Purpose

A lentivirus vector to integrate a surface membrane anchored human EPO gene

Depositor

Tao Liu

Article

Liu et al Cell Chem Biol. 2017 Jun 22;24(6):767-77

Insert

erythropoietin(EPO) (EPO Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q

Plasmid

#132545

Purpose

Expresses CIRTS-8 (bdefensin 3-TBP6.7-hADAR(299-701)E488Q) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q-NLS

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

cmv d0

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
3xFlag-HaloTag-EZH2 HDR - pDY053

Plasmid

#171108

Purpose

Homology directed repair plasmid for generating HaloTag-EZH2 cells

Depositor

Thomas Cech

Article

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

EZH2 (EZH2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAX-EGFP-CIP4

Plasmid

#178363

Purpose

Expresses CIP4 c-terminal labeled with EGFP in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Cdc42 Interacting Protein 4 (TRIP10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV-PGKpuro2ABFP

Plasmid

#122372

Purpose

Expresses puro-2A-BFP in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

Puro-2A-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)

Plasmid

#156463

Purpose

Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM

Depositor

Andre Stiel

Article

Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

Inserts

Heme Oxygenase
ReBphP-PCM

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HCMVgL-FLAG (TB40/E)

Plasmid

#136449

Purpose

Mammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tag

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein L (UL115 Human betaherpesvirus 5)

Use

Tags

Short SGGSG linker and FLAG tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV.PGK.mLmx1a

Plasmid

#33013

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

LIM homeobox transcription factor 1 alpha (Lmx1a Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
SRSF8_RRM_pGEX

Plasmid

#135119

Purpose

Recombinant expression of SRSF8 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SRSF8 (SRSF8 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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