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Showing: 501 - 543 of 543 results

  4. pDyn1

    Plasmid
    #64067
    Purpose
    Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).
    Depositor
    Andrew Carter
    Article
    Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:
    Insert
    DYNC1H1 (DYNC1H1 Human)
    Use
    Cre/Lox
    Tags
    His-ZZ-TCS-SNAPf
    Expression
    Insect
    Mutation
    Codon optimized for Sf9
    Promoter
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    64067-plasmid-map-sequence-id-107133

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  7. TFORF0208

    Plasmid
    #142556
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXP2 (FOXP2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    142556-plasmid-map-sequence-id-427312

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  8. TFORF0210

    Plasmid
    #142557
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXP2 (FOXP2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142557-plasmid-map-sequence-id-395974

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  11. TFORF1117

    Plasmid
    #143930
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BRIP1 (BRIP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143930-plasmid-map-sequence-id-404592

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  15. TFORF0209

    Plasmid
    #141556
    Purpose
    Lentiviral vector for overexpressing the FOXP2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXP2 (FOXP2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141556-plasmid-map-sequence-id-276804

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  18. TFORF0207

    Plasmid
    #141555
    Purpose
    Lentiviral vector for overexpressing the FOXP2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXP2 (FOXP2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141555-plasmid-map-sequence-id-276801

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  32. pDONR221-SLC25A46_STOP

    Plasmid
    #161137
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC25A46 (SLC25A46 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161137-plasmid-map-sequence-id-413584

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  36. pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)

    Plasmid
    #227608
    Purpose
    Inducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Use
    Tags
    10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    aa 2-83 deleted
    Promoter
    AOX1
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227608-plasmid-map-sequence-id-455573

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  39. pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)

    Plasmid
    #227609
    Purpose
    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Use
    Tags
    10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-544, 651-757 only [TM deleted]
    Promoter
    AOX1
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227609-plasmid-map-sequence-id-455572

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  40. pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)

    Plasmid
    #227611
    Purpose
    Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Use
    Tags
    MBP, 10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-88 only
    Promoter
    AOX1
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227611-plasmid-map-sequence-id-455570

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  41. pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

    Plasmid
    #227610
    Purpose
    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Use
    Tags
    10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-400, 706-757 [TM and HB2 deleted]
    Promoter
    AOX1
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227610-plasmid-map-sequence-id-455569

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  43. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

    Plasmid
    #111903
    Purpose
    Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.
    Depositor
    Andrew Carter
    Inserts
    His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
    DYNC1I2 (DYNC1I2 Human)
    DYNC1LI2 (DYNC1LI2 Human)
    DYNLT1 (DYNLT1 Human)
    DYNLRB1 (DYNLRB1 Human)
    DYNLL1 (DYNLL1 Human)
    Use
    Tags
    8xHis, SNAPf-tag, TEV site, and ZZ-tag
    Expression
    Insect
    Mutation
    Optimised for expression in Sf9 cells.
    Promoter
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111903-plasmid-map-sequence-id-215307

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Showing: 501 - 543 of 543 results