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Plasmid#164832PurposemiHiFiCas9 expressing plasmidDepositorInsertmiHiFiCas9 (contains Brex27 at sequence 5031-5138).
UseCRISPRTagsFLAG and NLSExpressionMammalianMutationchange R 691 to APromoterCMVAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTO135_pENO1-CaCas9-tCyc1
Plasmid#171103PurposeExpresses Cas9 in Candida aurisDepositorInsertCaCas9
UseTagsExpressionYeastMutationPromoterENO1Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBFv-U6.2
Plasmid#138400PurposegRNA expression vector for DrosophilaDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterU6-2Available sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_PLCG1:1-1291
Plasmid#211089PurposeMammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.DepositorInsertPLCG1:M1-L1291 (PLCG1 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationisoform 2. I813T natural variant (VAR_011908)PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSFVCs-lacZ
Plasmid#92076PurposeThis is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.DepositorInsertC-lacZ
UseC-lacz expression plasmid, for run-off transcript…TagsExpressionMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-mTagBFP2-2A
Plasmid#102602PurposeControl lentivector. Coexpression of mTagBFP and gene of interest from CMV promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
FokI-mCherry-LacI-NLS
Plasmid#215096PurposeExpresses FokI-mCherry-LacI-NLS in mammalian cells.DepositorInsertFokI-mCherry-LacI-NLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
EMTB-mScarletI
Plasmid#137801PurposeVisualization of microtubulesDepositorArticleInsertEnsconsin microtubule binding domain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT29
Plasmid#138521Purposephage cloningDepositorInsertphage genes
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDY0310 TP901-1 Integrase
Plasmid#179110PurposeTP901-1 Integrase expression vectorDepositorInsertTP901-1 Integrase
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-mito-mEm
Plasmid#185596PurposeLentiviral construct for expression of mEmerald targeted to mitochondriaDepositorInsertmito-mEm
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCali2
Plasmid#86119PurposeMultiplex temperature-gated expression of mWasabi and mCherry reportersDepositorInsertsTlpA36
mWasabi
TcI
mCherry
UseTags6xHisExpressionBacterialMutationA67T and D341A, P60L, D135V, K187R, K202I, L208QPromoterpR/pL, pR/pL, pLacI, pTlpA, and pTlpA, pLacIAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTO128_pPZP-NATca
Plasmid#171105PurposeA. tumefaciens TI plasmid encoding NATca in T-DNADepositorInsertNAT (Nourseothricin Resistance)
UseA. tumefaciens ti plasmidTagsExpressionMutationPromoterAgTEF2Available sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA
Plasmid#140220PurposeConstitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activatorDepositorInsertdLbCpf1
UseCRISPRTagsminiVPR-mCherryExpressionMammalianMutationD832APromoterPGKAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt1 I367T IRES mRuby3
Plasmid#170642PurposeExpression of syt1 in cultured neurons by lentiviral transductionDepositorInsertSYT1 (Syt1 Rat)
UseLentiviralTagsExpressionMutationI367TPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmiCas9
Plasmid#162758Purposeexpress miCas9DepositorInsertmiCas9
UseCRISPRTagsFLAG and NLSExpressionMammalianMutationContains Brex27 sequence range is 4958-5065PromoterCMVAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-69: NPM1-mTagRFP-T
Plasmid#124608PurposeHomology arms and linker-mTagRFP-T sequence for C-terminus tagging of human NPM1DepositorInsertNPM1 Homology Arms with linker-mTagRFP-T (NPM1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_MFSD2A
Plasmid#131893PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertMFSD2A (MFSD2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
GPR39-Tango
Plasmid#66357PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPR39 (GPR39 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST
Plasmid#208038PurposeEnables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidinDepositorInsertPML (isoform IVa) (PML Human)
UseLentiviralTagsFLAG, N-terminal Split-TurboIDExpressionMutationPromoterEFSAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH41744
Plasmid#48017DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGSHU
Plasmid#72244PurposeContains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
hyg
I-SceI
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNTI661 pRPR1(TetO)-sgRNA
Plasmid#139475PurposeVector for tet-regulated sgRNA expression in yeastDepositorInsertsingle guide RNA scaffold
UseCRISPRTagsExpressionYeastMutationPromoterpRPR1(TetO)Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
gRNA Cloning Vector Bbs I ver. 2
Plasmid#85586PurposeAn empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mouse cdk6 wt
Plasmid#75170PurposeMammalian expression of mouse cdk6DepositorInsertcyclin dependent kinase 6 (Cdk6 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b
Plasmid#184565PurposeInducible expression of PbuCas13b nuclease in bacteria.DepositorInsertCas13b nuclease from Prevotella buccae
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBI-eGFP-Rab31
Plasmid#200265PurposeExpression of eGFP-Rab31 (human) in mammalian cellsDepositorInsertRab31 (human cDNA) (RAB31 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBXD8-Strep-HA
Plasmid#113477PurposeExpression of human UBXD8 with C-terminal strep-HA tagDepositorInsertUBXD8 (FAF2 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPGxRF3
Plasmid#138384PurposeT2A-GAL4 knock-in cassetteDepositorInsertT2A-GAL4
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
TAAR2-Tango
Plasmid#66509PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertTAAR2 (TAAR2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-eDHFR-MCS
Plasmid#172108PurposeMammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFRDepositorInsertEGFP-eDHFR-20aa (folA E. coli)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV_ADRB2-mTurquoise2-iLID
Plasmid#161002PurposeMammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusionDepositorInsertADRB2-mTurquoise2-iLID
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLDPuro-hsnSR100N
Plasmid#35172DepositorInsertSRRM4 (SRRM4 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
hSYN-mito-dsRED2
Plasmid#173069Purposemitochondria targeted dsRED2 expressed off of human Synapsin I promoter.DepositorInsertMito
UseLentiviralTagsdsREDExpressionMammalianMutationPromoterhuman synapsin 1Available sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet
Plasmid#130509Purposeexpresses mScarletDepositorInsertmScarlet
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-beta2AR
Plasmid#106063PurposeExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-beta2AR; Opto-beta2AR; F5; 716)DepositorInsertOpto-beta2AR
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2398
Plasmid#142066PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZFPM2 (ZFPM2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_NPM1_G6
Plasmid#178091PurposeExpresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.DepositorInsertNPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET48 AtGUN1-PS
Plasmid#136358PurposeBacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 918)DepositorInsertcDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918 (GUN1 Mustard Weed)
UseTagsTRX-HisExpressionBacterialMutationPromoterT7Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only