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pHR_Gal4UAS_4D5-High-CAR-mCherry_PGK_4D5-Low-SynNotch_Gal4VP64_RHL133

Plasmid

#164822

Purpose

Lentiviral vector for Gal4 inducible AntiHER2 4D5-High CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Inserts

AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

miniCMV and pGK

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSGAb-km

Plasmid

#121999

Purpose

A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

Depositor

Quanjiang Ji

Article

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Insert

none

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA GFP10-ZIP

Plasmid

#182241

Purpose

Tripartite split-GFP mammalian. Express leucine zipper with GFP10

Depositor

Stéphanie Cabantous

Article

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

GFP10-ZIP

Use

Tags

split-GFP10 tripartite

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA ZIP-GFP11

Plasmid

#182242

Purpose

Tripartite split-GFP mammalian. Express leucine zipper with GFP11

Depositor

Stéphanie Cabantous

Article

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

ZIP-GFP11

Use

Tags

split-GFP11 tripartite

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pWPT-GFP

Plasmid

#12255

Depositor

Didier Trono

Insert

EGFP

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pOpen-P45

Plasmid

#165570

Purpose

Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.

Depositor

FreeGenes Project, Jennifer Molloy, Drew Endy

Insert

P45

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pTP2620

Plasmid

#113311

Purpose

human Rad50 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

Insert

Rad50 (RAD50 Human)

Use

Tags

HA, 6xhis

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTP813

Plasmid

#113308

Purpose

human Mre11 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Bhaskara et al Mol Cell. 2007 Mar 9;25(5):647-61. doi:

Insert

Mre11 (MRE11 Human)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-MBP-2xNLS-tdTomato

Plasmid

#104054

Purpose

An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-tdTomato

Use

AAV

Tags

NLS

Expression

Mutation

Promoter

MBP

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA Scramble

Plasmid

#162011

Purpose

Lentiviral negative control vector containing scramble shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

scramble shRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTP284

Plasmid

#113312

Purpose

human Nbs1 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Myler et al Mol Cell. 2017 Sep 7;67(5):891-898.e4. d

Insert

Nbs1 (NBN Human)

Use

Tags

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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IF-GFP-ATRX

Plasmid

#45444

Purpose

ATRX expression vector

Depositor

Michael Dyer

Article

Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

Insert

ATRX (ATRX Mouse, Human)

Use

Tags

GFP and HA

Expression

Bacterial and Mammalian

Mutation

isoform 2, missing codon E124

Promoter

PCMV

Available since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6a

Plasmid

#207851

Purpose

Mammalian expression of PE6a prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6a

Use

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Ai9

Plasmid

#22799

Depositor

Hongkui Zeng

Article

Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

Insert

CAG-Floxed tdTomato

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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FLAG-TREX1

Plasmid

#27218

Depositor

Judy Lieberman

Article

Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

Insert

TREX1 (TREX1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

n/a

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pCasAb-apr

Plasmid

#121998

Purpose

Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

Depositor

Quanjiang Ji

Article

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSD141B

Plasmid

#218139

Purpose

Lentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD56-eGFP-IRES2-mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV CreERT2 puro

Plasmid

#22776

Depositor

Tyler Jacks

Article

Kumar et al Genes Dev. 2009 Nov 10. ():.

Insert

CreERT2

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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pCMU-TPr

Plasmid

#61189

Purpose

Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtTIP1-1

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

AtUBQ10

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCMU-NUCr

Plasmid

#61168

Purpose

Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

NLS-mCherry-GUS

Use

Tags

Expression

Plant

Mutation

Promoter

AtUBQ10

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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