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Plasmid#89727PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
6-His-MBP-TEV-FnCpf1
Plasmid#90094PurposeBacterial expression plasmid for protein purificationDepositorInsertFnCpf1 (humanized)
UseTags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(+6)
Plasmid#89728PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a longer versi…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(-6)
Plasmid#89730PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-6) truncated by 6 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a shorter vers…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(-12)
Plasmid#89731PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-12) truncated by 12 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a shorter vers…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(-18)
Plasmid#89732PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-18) truncated by 18 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a shorter vers…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(-3)
Plasmid#89729PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-3) truncated by 3 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a shorter vers…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-BrCas12b-RFND
Plasmid#195339PurposeExpresses Engineered BrCas12b in BacteriaDepositorInsertBrCas12b
UseCRISPR and Synthetic BiologyTags6xHis and 6xHis, MBP, TEV cleavage siteExpressionBacterialMutationR160E, F208W, N524V, D868VPromoterT7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-BrCas12b-FNLDTA
Plasmid#195340PurposeExpresses Engineered BrCas12b in BacteriaDepositorInsertBrCas12b
UseCRISPR and Synthetic BiologyTags6xHis Tag and MBP, TEV cleavage site, 6x His TagExpressionBacterialMutationF208W, N524V, L795I, D868V, T874S, A1015EPromoterT7 PromoterAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pR2HYG
Plasmid#30170DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagsdsRed2ExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNS2
Plasmid#50610PurposeNS module plasmid for Cermak, et al., 2011 TALEN kitDepositorInsertNS1
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNS8
Plasmid#50616PurposeNS module plasmid for Cermak, et al., 2011 TALEN kitDepositorInsertNS8
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNS9
Plasmid#50617PurposeNS module plasmid for Cermak, et al., 2011 TALEN kitDepositorInsertNS9
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNS10
Plasmid#50618PurposeNS module plasmid for Cermak, et al., 2011 TALEN kitDepositorInsertNS10
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNS1
Plasmid#50609PurposeNS module plasmid for Cermak, et al., 2011 TALEN kitDepositorInsertNS1
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNS3
Plasmid#50611PurposeNS module plasmid for Cermak, et al., 2011 TALEN kitDepositorInsertNS3
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNS4
Plasmid#50612PurposeNS module plasmid for Cermak, et al., 2011 TALEN kitDepositorInsertNS4
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNS5
Plasmid#50613PurposeNS module plasmid for Cermak, et al., 2011 TALEN kitDepositorInsertNS5
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNS6
Plasmid#50614PurposeNS module plasmid for Cermak, et al., 2011 TALEN kitDepositorInsertNS6
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNS7
Plasmid#50615PurposeNS module plasmid for Cermak, et al., 2011 TALEN kitDepositorInsertNS7
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only