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Plasmid#165052PurposeIntegrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiaeDepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID5Z
Plasmid#165047PurposeIntegrative expression of AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…PromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
AD59_pEP.DonorCLYBL.TS
Plasmid#199227PurposeDonor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locusDepositorInsertExpression unit for PuroR.T2A.EGFP selectable and reporter markers
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAID5XY
Plasmid#165051PurposeIntegrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…PromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-PGK.Pro
Plasmid#79489PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) Human PGK promoterDepositorInsertPGK Promoter (PGK1 Human, Synthetic)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterPGKAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYN2_1
Plasmid#184757PurposePlasmid for genome editing by CRISPR/Cas9DepositorInsertsCas9
sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
UseSynthetic BiologyTags2xNLSExpressionBacterial and YeastMutationPromoterPGK1 and tRNA-Phe-HDV RibozymeAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEM.P03R
Plasmid#198646PurposeEasy-MISE toolkit pEM-plasmid containing PGK1 promoter with FG protruding endsDepositorInsertpPGK1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P03L
Plasmid#198645PurposeEasy-MISE toolkit pEM-plasmid containing PGK1 promoter with EF protruding endsDepositorInsertpPGK1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB m Rosella I
Plasmid#71247Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.DepositorInsertRosella
UseTagsyeast mitochondrial targeting sequence of citrate…ExpressionYeastMutationPromoterPGK1Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
BB44_pmc.DonorR5.TS
Plasmid#199223PurposeDonor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locusDepositorInsertExpression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only