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Plasmid#226306PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with C to S CsoS2 MR1-5
UseTagsExpressionBacterialMutationC292S, C310S, C351S, C369S, C467S, C485S, C526S, …PromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB46
Plasmid#226305PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with C to S CsoS2 MR1-4
UseTagsExpressionBacterialMutationC292S, C310S, C351S, C369S, C467S, C485SPromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB45
Plasmid#226304PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with C to S CsoS2 MR1-2
UseTagsExpressionBacterialMutationC292S, C310S, C351S, C369SPromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBM2
Plasmid#225289PurposeE. coli-Campylobacter shuttle vector; KanRDepositorInsertReplication elements from pCC311 for pJBM2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBM3
Plasmid#225290PurposeE. coli-Campylobacter shuttle vector; KanRDepositorInsertReplication elements from pCC388 for pJBM3
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAP72_MS2
Plasmid#220623PurposeCassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.DepositorTypeEmpty backboneUseShuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)
Plasmid#218640PurposeGateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)DepositorInsertNLRP3 (Nlrp3 Mouse)
UseGateway entryTagsExpressionMutationdelPYD (I125M-END)PromoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 hNLRP3 R262W (disease-associated mutation)
Plasmid#218647PurposeGateway shuttling vector for cloning NLRP3-R262W (no stop codon)DepositorInsertNLRP3 (NLRP3 Human)
UseGateway entryTagsExpressionMutationR262WPromoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 msNLRP3(ΔPYD, I125M-END)
Plasmid#218641PurposeGateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)DepositorInsertNLRP3 (Nlrp3 Mouse)
UseGateway entryTagsExpressionMutationdelPYD (I125M-END)PromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMGA
Plasmid#139931PurposeM. magneticum/E. coli shuttle vector (pMGT and p15A ori)DepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23119Available sinceMarch 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_HD_G1333-DddA11'-C
Plasmid#199797PurposeptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NG_G1333-DddA11'-C
Plasmid#199798PurposeptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NI_G1333-DddA11'-C
Plasmid#199799PurposeptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NN_G1333-DddA11'-C
Plasmid#199800PurposeptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_HD_G1333-DddA11'-N
Plasmid#199801PurposeptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NG_G1333-DddA11'-N
Plasmid#199802PurposeptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NI_G1333-DddA11'-N
Plasmid#199803PurposeptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NN_G1333-DddA11'-N
Plasmid#199804PurposeptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseTalenTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_HD_G1397-DddA11'-N
Plasmid#191611PurposeptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only