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Showing: 2161 - 2180 of 7894 results

  1. BPK1520

    Plasmid
    #65777
    Purpose
    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SpCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65777-plasmid-map-sequence-id-316938

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  4. Biomolecular FeedBack (FB)

    Plasmid
    #109391
    Purpose
    This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    pJ23100mut-dCas9-T- phtpG1-sgRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109391-plasmid-map-sequence-id-210784

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  12. pJRH051

    Plasmid
    #171625
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171625-plasmid-map-sequence-id-335505

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  14. pRDA_526

    Plasmid
    #184859
    Purpose
    Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    184859-plasmid-map-sequence-id-362663

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  18. pX330-PITCh-LMNB1

    Plasmid
    #207770
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Insert
    sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    207770-plasmid-map-sequence-id-416199

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Showing: 2161 - 2180 of 7894 results