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pcDNA3-PPO-Venus

Plasmid

#139502

Purpose

Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Use

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CaMPARI V398D

Plasmid

#60786

Purpose

Expresses CaMPARI V398D in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Loren Looger, Eric Schreiter

Article

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Insert

CaMPARI V398D

Use

Tags

Nuclear Export Signal

Expression

Mammalian

Mutation

V398D

Promoter

CMV

Available since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin WT

Plasmid

#50853

Purpose

expresses human cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 HisC EXO1b

Plasmid

#68268

Purpose

Mammalian expression of human EXO1

Depositor

Stefano Ferrari

Article

El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

Insert

EXO1 (EXO1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

b form, codons 1-846. Please see depositor commen…

Promoter

CMV

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jnk3a2

Plasmid

#13759

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a2 (MAPK10 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO/GFP-TTP

Plasmid

#141190

Purpose

Expresses GFP-TTP in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

TTP (ZFP36 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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Nano-lantern/pcDNA3

Plasmid

#51970

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v1-Fc(IgG1)

Plasmid

#145164

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity variant 1

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RaACR_687-EYFP

Plasmid

#111077

Purpose

Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RaACR_687

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-EKARev(T/A)

Plasmid

#118475

Purpose

Negative-control mutant for RAB-EKARev biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev(T/A)

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB6a

Plasmid

#19529

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJB6a (DNAJB6 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pCDNA3-C1-mCherry-LOVPLD

Plasmid

#219559

Purpose

ER-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

C1-mCherry-LOVPLD

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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ABCG1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176944

Purpose

Mammalian expression vector encoding ABCG1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

ABCG1 (ABCG1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Golgi-ZapCY1

Plasmid

#36322

Depositor

Amy Palmer

Article

Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

Insert

Golgi-ZapCY1 high affinity Zn(II) sensor

Use

Tags

GalT (first 60 amino acids)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-mTagBFP2-MRTF-B

Plasmid

#182062

Purpose

Mammalian expression vector encoding myocardin-related transcription factor B tagged with mTagBFP2.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mTagBFP2-MRTF-B (Mrtfb Mouse)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-mRFP-pcDNA3.1-

Plasmid

#52510

Purpose

expresses mRFP in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

mRFP

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

engineered variant of tetrameric red fluorescent …

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-dyskerin

Plasmid

#126870

Purpose

Expresses 3xFlag dsykerin

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

Dyskerin

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HCMVgH (TB40/E)

Plasmid

#136446

Purpose

Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-length

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein H (UL75 Human betaherpesvirus 5)

Use

Tags

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp12

Plasmid

#157709

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp12 (ORF1ab SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-HIRA-polyA

Plasmid

#59781

Purpose

Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

HIRA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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