Addgene

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ShadowG-SUMO1

Plasmid

#175239

Purpose

Bacterial expression and purification, conjugatable SUMO for SUMOylation

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

SUMO1 (SUMO1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBad-Xis/Int Reset Flipper

Plasmid

#38207

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

Bxb1-Excisionase
Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Tags

Expression

Mutation

Promoter

pBAD

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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BiP-mGFP P495L

Plasmid

#62232

Purpose

Mutant of hamster BiP-mGFP unable to bind clients.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mGFP-KDEL

Use

Tags

mGFP

Expression

Mammalian

Mutation

Promoter

cmv

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-HA::UPRT

Plasmid

#47110

Purpose

for subcloning UPRT into expression vectors

Depositor

Chris Doe, Mike Cleary

Article

Miller et al Nat Methods. 2009 Jun;6(6):439-41. doi:

Insert

uracil phosphoribosyltransferase

Use

Tags

HA

Expression

Mutation

Promoter

Available since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pET-pro-siRNA-V2

Plasmid

#111086

Purpose

For production of recombinant siRNAs (pro-siRNAs) in E. coli.

Depositor

Linfeng HUANG

Article

Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

Type

Empty backbone

Use

RNAi

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pSIM24 KanR

Plasmid

#67583

Purpose

Plant Gene Expression Vector

Depositor

Indu Maiti

Article

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

M24

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-CFP

Plasmid

#90103

Purpose

CFP control expression plasmid

Depositor

Samuel Miller

Article

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

CFP

Use

Broad-host range

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pYZ462

Plasmid

#187970

Purpose

TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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hRIP3 GFP wt

Plasmid

#41387

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (RIPK3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 Rheb1

Plasmid

#19312

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RHEB Ras homolog enriched in brain (RHEB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pC1-CMV-DAAO-NES

Plasmid

#141107

Purpose

Expresses DAAO with nuclear export signal in mammalian cells

Depositor

Vsevolod Belousov

Article

Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

Insert

DAAO-NES

Use

Tags

Nuclear export signal

Expression

Mammalian

Mutation

deleted DAAO amino acids 367-368

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD_Kan-LacI

Plasmid

#79826

Purpose

Arabinose induced expression of Lac repressor

Depositor

Katja Arndt

Article

Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

Insert

LacI

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

araBp

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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Flag Rab12 wild type

Plasmid

#86787

Purpose

mammalian expression of Rab12

Depositor

Peter McPherson

Article

Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

Insert

Rab12 (RAB12 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pX330S-4

Plasmid

#58780

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-MPAct-mRuby3-IRES-Puro

Plasmid

#155231

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mRuby sensor

Use

Lentiviral

Tags

mRuby3

Expression

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-Lb-crRNA

Plasmid

#78957

Purpose

Cloning vector for expression of LbCpf1 crRNA. It contains BsmB1 site for cloning.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pmr101A-hVPS26

Plasmid

#17636

Depositor

James Hurley

Article

Shi et al Nat Struct Mol Biol. 2006 Jun . 13(6):54

Insert

VPS26 (VPS26A Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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pKIKOlacZCm

Plasmid

#46764

Purpose

E.coli genomic integration at lacZ

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TrxRFP1

Plasmid

#98996

Purpose

mammalian expression of a biosensor to monitor thioredoxin redox dynamics

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP

Use

Tags

His-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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Gata4-GFP donor

Plasmid

#113115

Purpose

Donor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Gata4 donor region including homology arms and eGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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