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Plasmid#115520PurposeTo construct multiple sgRNA expression vector for CRISPRDepositorInsertsgRNA scaffolf-U6 promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUCas9Cyan
Plasmid#85554PurposeExpresses Cas9 with fluorescent Cyan reporterDepositorInserthumanised S.pyrogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterhUBCAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGGA_RT_Sa_Cas9
Plasmid#136962PurposeGoldengate/Greengate module for genome editing, using the SaCAS9.DepositorInsertSaCAS9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORFE1001
Plasmid#112079PurposeOverexpression of the AtCAS9 in plant under 2x35S promoterDepositorInsertAtCAS9
UseCRISPRTagsExpressionPlantMutationPlant codon optimized Cas9 S. pyogenesPromoter2x35SAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORFE0002
Plasmid#112071PurposeAtCAS9 nickase D10A module CDS1DepositorInsertAtCAS9 nickase D10A
UseCRISPRTagsExpressionPlantMutationPlant codon optimized nCas9 D10A S. pyogenesPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORFE0003
Plasmid#112072PurposeAtDeadCAS9 D10A H840A module CDS1DepositorInsertAtDeadCAS9 D10A H840A
UseCRISPRTagsExpressionPlantMutationPlant codon optimized dCas9 D10A S. pyogenesPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEAD1.1.0-gDNA
Plasmid#112425PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TEAD1DepositorInsertTEAD1 (TEAD1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgRosa26
Plasmid#159914PurposeMutagenesis of Rosa26 with SauCas9DepositorInsertRosa26 gRNA (Gt(ROSA)26Sor Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgTh
Plasmid#159901PurposeMutagenesis of ThDepositorInsertTh (Th Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2
Plasmid#199486PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertGal4vp16/4xnrUAS-mTagBFP2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2
Plasmid#199485PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertGal4vp16/4xnrUAS-mTagBFP2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMLS287
Plasmid#73730PurposeSapTrap flexible linker C-terminal connector donor plasmidDepositorInsertFlexible Linker
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMLS279
Plasmid#73729PurposeSapTrap FLP-on C-terminal connector donor plasmidDepositorInsertC-terminal FLP-on
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMLS288
Plasmid#73735PurposeSapTrap flexible linker N-terminal connector donor plasmidDepositorInsertFlexible Linker
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2
Plasmid#199482PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertGal4vp16/4xnrUAS-mKate2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNR4A1.1.0-gDNA
Plasmid#112391PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1DepositorInsertNR4A1 (NR4A1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1
Plasmid#184294PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1DepositorInsertsgRNA-Adrb1
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEXfrt-SaCas9-U6-sgRosa26
Plasmid#159915PurposeMutagenesis of Rosa26DepositorInsertRosa26 (Gt(ROSA)26Sor Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2
Plasmid#199484PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertGal4vp16/4xnrUAS-mKate2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2
Plasmid#199481PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertGal4vp16/4xnrUAS-mKate2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only