Showing: 2161 - 2180 of 4605 results
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Plasmid#213913PurposeEntry clone containing 3×FLAG-NLS-zCas9-NLS encoding sequence, Pisum sativum rbcS E9 terminator. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertzCas9
UseCRISPRTagsExpressionMutationZea mays codon-optimized Cas9PromoterAvailabilityAcademic Institutions and Nonprofits only -
pZE21/UBP1/ClpS_V65I
Plasmid#98567PurposeExpresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operonDepositorInsertsTruncated ubiquitin cleavase, codon optimized
Mutated ATP-dependent Clp protease adapter protein
UseTagsExpressionBacterialMutationContains the V65I mutation that increases discrim…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pOPINB-HOIL-1 helix-RBR (233–510)
Plasmid#193859PurposeExpression of human His-tagged HOIL-1 (RBCK1) helix-RBR (aa 233–510) codon optimized for E. coliDepositorInsertHOIL-1 (RBCK1 Human)
UseTags3C protease cleavage site and 6x-His tagExpressionBacterialMutationResidues 233–510, codon optimised for expression …PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
ATX3-77Q
Plasmid#184249PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertAtaxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Synthetic, Human)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationCodon optimization for protein expression in BL2…PromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
ATX3-77Q-R388G
Plasmid#184251PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Synthetic, Human)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationC-terminal codon optimization for protein expres…PromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
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His-ZZ-TEV-SNAPf-DYNC2H1
Plasmid#132524PurposeFull length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragmentsDepositorInsertDYNC2H1 (DYNC2H1 Human)
UseTags8x His, Linker-TEV-linker-TEV, Precission cleavag…ExpressionInsectMutationCodon optimised for Sf9 expressionPromoterAvailabilityAcademic Institutions and Nonprofits only -
miABE
Plasmid#205412PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoterDepositorInsertpU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
UseTagsExpressionMammalianMutationD61A+E85R+H369R+S387R+S457RPromoterhU6, CAG, CMVAvailabilityAcademic Institutions and Nonprofits only -
pSG5-ERRbeta2
Plasmid#52186PurposeExpresses human ERRbeta2 splice variant in mammalian cellsDepositorInsertEstrogen-related receptor beta (ESRRB Human)
UseTagsExpressionMammalianMutationwild type sequence; codon optimized to exclude Ec…PromoterSV40AvailabilityAcademic Institutions and Nonprofits only -
pACT2-CAS9c
Plasmid#51055PurposeExpress Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.DepositorInsertCas9c
UseCRISPRTagsSV40 NLSExpressionBacterial and YeastMutationPromoteryeast ADH1 promoterAvailabilityAcademic Institutions and Nonprofits only -
pSQT1601
Plasmid#53369Purposehuman codon optimized Csy4 and FokI-dCas9 expression plasmidDepositorInserthCsy4-T2A-NLS-hFokI-dCas9-NLS
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-human lyn–GFP
Plasmid#35958PurposeExpresses human lyn protein codon optimized for zebrafish expression.DepositorInsertLYN (LYN Human)
UseTagsEGFPExpressionMammalianMutationwtPromotercmvAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-human lyn(C468A)–GFP
Plasmid#35959PurposeExpresses human lyn protein codon optimized for zebrafish expression.DepositorInsertLYN (LYN Human)
UseTagsEGFPExpressionMammalianMutationc468aPromotercmvAvailabilityAcademic Institutions and Nonprofits only -
pMSP2
Plasmid#26282DepositorInsertMSP2
UseTags6-HisExpressionBacterialMutationsynthetic gene of dimer of deletion mutant (1-43)…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA6 MCV LTco
Plasmid#40200DepositorInsertMCPyV LT codon optimized
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA6 MCV sTco
Plasmid#40201DepositorInsertMCPyV sT codon optimized
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pME-Cas9
Plasmid#63154PurposeMiddle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLSDepositorInsertCas9
UseCRISPR; Tol2 middle entry vector for gatewayTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_2
Plasmid#60574Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_2
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_3
Plasmid#60575Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_3
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_4
Plasmid#60576Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_4
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailabilityAcademic Institutions and Nonprofits only
Showing: 2161 - 2180 of 4605 results