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Plasmid#165971PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET32-sLatherin
Plasmid#61685Purposebacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusionDepositorInsertLatherin (LATH horse)
UseTagsHIS and Trx-Tag thioredoxin proteinExpressionBacterialMutationCodon optimized for E.coliPromoterT7Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-hCasD10A-pA
Plasmid#51638PurposeExpresses Cas9-nickase in mammalian cells and zygotes.DepositorInsertCodon-optimized Cas9 D10A
UseCRISPRTagsFLAG, NLS, and polyAExpressionMammalianMutationPromoterCAG promoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pprm1-luciferase; his-3
Plasmid#137728PurposeCommunication-activated luciferase in Neurospora crassaDepositorInsertsLuciferase
Pprm-1 Promoter
UseLuciferase; Neurospora crassa expressionTagsExpressionMutationCodon-optimized for N. crassaPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS-HA-B3
Plasmid#51270PurposeExpression of the site-specific recombinase B3 (mouse codon-optimized)DepositorInsertNLS-HA-B3
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
PARP15-c021
Plasmid#173102PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPARP15 (PARP15 Human)
UseTagsmCeruleanExpressionBacterialMutationG287-N470, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-100RPU.2-coEPO-lox2272
Plasmid#154828PurposeDonor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietinDepositorInsertcoEPO
UseCre/LoxTagsExpressionMammalianMutationCodon-optimized (Cricetulus griseus)Promoter100RPU.2Available sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p33Fn
Plasmid#158155PurposeExpression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationRice codon optimizedPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ155
Plasmid#69306PurposeGateway entry vector with AteCas9D10ADepositorInsertAteCas9D10A (Arabidopsis codon optimized)
UseCRISPRTagsExpressionMutationD10A mutation; NLS fusionPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
p32Fn
Plasmid#158153PurposeConstruct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and LbCpf1, respectively. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationRice codon optimizedPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p415TEF cyto roGFP2-Grx2(yeast)
Plasmid#65005PurposeYeast expression plasmid for roGFP2-Grx2; yeast codon optimized roGFP; yeast Grx2DepositorInsertGRX2
UseTagsroGFP2ExpressionYeastMutationPromoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
p1148-pCSDest-AcrIIA4-mCherry
Plasmid#126204PurposeMammalian expression of AcrIIA4 fused to mCherryDepositorInsertcodon-optimized AcrIIA4
UseTagsFLAG/NLS and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSN074
Plasmid#166731PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS
UseCRISPRTagsSV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV rev
Plasmid#101339PurposeLTR drives GFP and internal SFFV promoter drives codon optimized revDepositorInsertGFP, rev
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]
Plasmid#168173PurposeExpression of BeCyclOp in BWMs of C. elegansDepositorInsertBeCyclOp (codon optimized for C. elegans)
UseTagsExpressionWormMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK415-phlTA2-1E
Plasmid#165970PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTG56A -myc
Plasmid#107459PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G56A conferring wildtype phenotypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationG56APromoterCMVAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV tat
Plasmid#101338PurposeLTR drives GFP and internal SFFV promoter drives codon optimized tatDepositorInsertGFP, tat
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSN069
Plasmid#166728PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-Mxi1
UseCRISPRTagsMxi1ExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
BlatCas9
Plasmid#163792PurposeThe plasmid expresses human codon-optimized BlatCas9 and gRNA expression elements.DepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only