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pIG-TAT-Xph20-ETWV

Plasmid

#133040

Purpose

Expresses TAT-Xph20-ETWV in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph20-ETWV

Use

Tags

His8, TAT, and TEV protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-LB2O

Plasmid

#188036

Purpose

Fusion of human MYC Transactivation Domain (TAD) MYC Box II (MBII) with human OCT4 protein expressed in pMXs vector

Depositor

Young Tang

Article

Wang et al Stem Cell Res. 2017 Dec;25:88-97. doi: 1

Insert

MYC TAD MBII region fused with OCT4 (POU5F1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-M2O

Plasmid

#188034

Purpose

Fusion of human MYC Transactivation Domain (TAD) MYC Box I/II (MBI/II) with human OCT4 protein expressed in pMXs vector

Depositor

Young Tang

Article

Wang et al Stem Cell Res. 2017 Dec;25:88-97. doi: 1

Insert

MYC TAD MBI/II region fused with OCT4 (POU5F1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pKnockinDonor-humanCTCF-miniAID-mClover3

Plasmid

#136883

Purpose

knockin donor vector of miniAID-mClover3 to C-terminus of endogenous human CTCF

Depositor

Chunliang Li

Article

Hyle et al Nucleic Acids Res. 2019 Jul 26;47(13):66

Insert

CTCF-knockin-homology arm-miniAID-mClover3

Use

Tags

miniAID-mClover3

Expression

Mammalian

Mutation

Promoter

no promoter

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-ER [TM(SAC1)]

Plasmid

#186572

Purpose

Expression of the Endoplasmic Reticulum marker mScarlet-ER [TM(SAC1)] in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

SAC1 (SACM1L Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C-terminal transmembrane region of the protein: 5…

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLI_C-tag TNF

Plasmid

#171178

Purpose

inducible expression of C-tag TNF

Depositor

Veit Hornung

Article

Pinci et al J Biol Chem. 2020 Dec 25;295(52):18065-1

Insert

C-tag TNF

Use

Lentiviral

Tags

C-tag (internal)

Expression

Mammalian

Mutation

ADAM cleavage site of TNF (AA73-76) was exchanged…

Promoter

tight TRE promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pRS316-SNU114

Plasmid

#111308

Purpose

pRS316-SNU114

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314-SNU114

Plasmid

#111309

Purpose

pRS314-SNU114

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 HA-SNU114

Plasmid

#111318

Purpose

HA-SNU114

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-A KD/MD

Plasmid

#104449

Purpose

Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

changed K94 to D and M96 to D

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-B KD/MD

Plasmid

#104450

Purpose

Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein B (VAPB Human)

Use

Tags

EGFP and HA

Expression

Mammalian

Mutation

changed K87 to D and M89 to D

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pT22gCfpUbm5En2E4PCh(#251)

Plasmid

#184083

Purpose

cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-chkEn2-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22Ubm5Eng2bE4PCh(#284)

Plasmid

#184088

Purpose

cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-ZfEng2b-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT22Ubm5Eng2aE4PCh(#283)

Plasmid

#184087

Purpose

cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-ZfEng2a-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-WT-VWF

Plasmid

#124794

Purpose

Expresses multimeric human VWF with C-terminal c-Myc tag

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF (VWF Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mCebpe

Plasmid

#79076

Purpose

Expression of mouse Cebpe

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Cebp epsilon (Cebpe Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pcdna3.1 hciap2mut

Plasmid

#8339

Depositor

Jon Ashwell

Article

Li et al Nature 2002 Mar 21;416(6878):345-7.

Insert

ciap2mut (BIRC3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

H574A

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pICSL30009

Plasmid

#50301

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

N terminal Myc tag (4x Myc)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad3 (1-145)

Plasmid

#14965

Depositor

Joan Massague

Article

Chen et al Cell. 2002 Jul 12. 110(1):19-32.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 1-145

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag-Smad3 (146-425)

Plasmid

#14966

Depositor

Joan Massague

Article

Chen et al Cell. 2002 Jul 12. 110(1):19-32.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 146-425

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only