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pMXs-IP-EGFP-ULK2

Plasmid

#38201

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

Unc-51 like kinase 2 (Ulk2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pgRNAkan-glpR

Plasmid

#169873

Purpose

Single guide RNA plasmid for Cas9 targeting glpR in P. putida

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

sgRNA towards glpR in Pseudomonas putida

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM-and1 in situ probe

Plasmid

#52102

Purpose

actinodin 1 and1 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 1 in situ probe (and1 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pMP4516

Plasmid

#107775

Purpose

Expression of ECFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-ECFP in pBBR1-mcs5

Use

Tags

pLacZ-ECFP

Expression

Bacterial

Mutation

Promoter

pLacZ

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCas5

Plasmid

#82378

Purpose

sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd1-1D4

Plasmid

#42263

Depositor

Jeremy Nathans, Chris Garcia

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 1 (Fzd1 Mouse)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP

Plasmid

#154303

Purpose

Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Inserts

histone H1
γ-tubulin

Use

Dictyostelium expression

Tags

mEGFP and mRuby3

Expression

Mutation

Promoter

actin15

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pL1P3 ZmUbiP:GRF-GIF:NosT

Plasmid

#198047

Purpose

MoClo Golden Gate Level 1 Position 3. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

Depositor

John Innes Centre - Wheat Transformation and Genome Editing, Cristobal Uauy

Insert

ZmUbiP::TaGRF4-GIF1::NosT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Zea mays (maize) ubiquitin promoter

Available since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd6-1D4

Plasmid

#42268

Depositor

Jeremy Nathans, Chris Garcia

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 6 (Fzd6 Mouse)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-mFzd10-1D4

Plasmid

#42272

Depositor

Jeremy Nathans, Chris Garcia

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 10 (Fzd10 Mouse)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pDS272

Plasmid

#34975

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2-GFP-LOVpep (MID2 Budding Yeast, A. sativa)

Use

Tags

Expression

Yeast

Mutation

T406A, T407A

Promoter

TEF

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pAL189

Plasmid

#22278

Purpose

Expresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

IntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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DAXX-myc

Plasmid

#1851

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

DAXX (Daxx Mouse)

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pDS271

Plasmid

#34974

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2-GFP-LOVpep (MID2 Budding Yeast, A. sativa)

Use

Tags

Expression

Yeast

Mutation

T406A, T407A, V416I

Promoter

TEF

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-IP-EGFP-ULK2(K39T)

Plasmid

#38206

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

Unc-51 like kinase 2 (Ulk2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed Lysine 39 to Threonine

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-flag-UTR

Plasmid

#183234

Purpose

Mammalian expression empty backbone

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Type

Empty backbone

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCas2F

Plasmid

#82377

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCG

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pQE9-His-p97deltaD2

Plasmid

#17229

Depositor

Hemmo Meyer

Article

Ramadan et al Nature. 2007 Dec 20. 450(7173):1258-62.

Insert

p97 (Vcp Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

changed codon 457 to stop codon

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
actc1b-actn2-mKate2

Plasmid

#109751

Purpose

Muscle specific zebrafish expression of mKate2 fused to alpha actinin 2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

alpha actinin 2

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS/Daxx myc S669A

Plasmid

#19282

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx myc S669A (Daxx Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only