Addgene

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tetO-TBX20

Plasmid

#170705

Purpose

doxycycline-inducible overexpression of TBX20

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

TBX20 (TBX20 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-SNAI2

Plasmid

#170702

Purpose

doxycycline-inducible overexpression of SNAI2

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

SNAI2 (SNAI2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7A-mGFP

Plasmid

#62633

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mGFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

Expression

Mammalian

Mutation

mGFP inserted in M3-M4 loop

Promoter

CMV

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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pTetOne-Six1-myc

Plasmid

#138530

Purpose

Inducible expression of Six1 with a myc tag

Depositor

Ana Teixeira, Christopher Grigsby

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

Six1 (Six1 Mouse)

Use

Tags

3x myc tag

Expression

Mammalian

Mutation

Promoter

TRE3Gs

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7A

Plasmid

#62284

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A)

Depositor

Henry Lester, Sherry Leonard

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

Expression

Mammalian

Mutation

partial duplication of CHRNA7

Promoter

CMV

Available since

March 3, 2015

Availability

Industry, Academic Institutions, and Nonprofits
AB.pCCL.sin.cPPT.U6.miR-431-Decoy.hPGK.GFP.WPRE

Plasmid

#46620

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-431 Decoy (MIR431, Mir431 Human, Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
tetO-TCF21

Plasmid

#170706

Purpose

doxycycline-inducible overexpression of TCF21

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

TCF21 (TCF21 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 Del AA 6

Plasmid

#184024

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of amino acids 689 to 694.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8 Del AA 6 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of amino acids 689 to 694

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 D-1

Plasmid

#184013

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of amino acids 685-706.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) D-1 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of amino acids 685-706.

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pUT1887-HQ858800

Plasmid

#27882

Depositor

Erich Grotewold, John Gray

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

ORPHAN transcription factor from DR828861

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7

Plasmid

#62276

Purpose

mammalian expression of human Alpha7 (CHRNA7)

Depositor

Henry Lester, Sherry Leonard

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRNA7 (CHRNA7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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tetO-SOX9

Plasmid

#170703

Purpose

doxycycline-inducible overexpression of SOX9

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

SOX9 (SOX9 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7-mGFP

Plasmid

#62629

Purpose

mammalian expression of human Alpha7 (CHRNA7) with mGFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

hAlpha7 (CHRNA7 Human)

Use

Tags

Expression

Mammalian

Mutation

mGFP inserted in M3-M4 loop

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCW-IRF4-Puro

Plasmid

#123323

Purpose

Lentiviral vector for expression of IRF4 under a Doxycycline inducible promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

IRF4 (IRF4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

several silent mutations to disrupt sgRNA targeti…

Promoter

Doxycycline inducible minimal CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7-mCherry

Plasmid

#62632

Purpose

mammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

hAlpha7 (CHRNA7 Human)

Use

Tags

Expression

Mammalian

Mutation

mCherry inserted into M3-M4 loop

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only
tetO-MITF

Plasmid

#170695

Purpose

doxycycline-inducible overexpression of MITF

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

MITF (MITF Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-MESP1

Plasmid

#170694

Purpose

doxycycline-inducible overexpression of MESP1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

MESP1 (MESP1 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_Ef1a:PE2-P2A-hMLH1dn

Plasmid

#184444

Purpose

Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

Depositor

Bobby Koeleman

Article

Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Ef1A

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7-YFP

Plasmid

#62630

Purpose

mammalian expression of human Alpha7 (CHRNA7) with YFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

hAlpha7 (CHRNA7 Human)

Use

Tags

Expression

Mammalian

Mutation

YFP inserted in M3-M4 loop

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 D-5

Plasmid

#184017

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 641 to 655.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) D-5 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of exon 19a (amino acids 696-701), and d…

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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