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Plasmid#133771Purposeexpress 6Xhis- SUMO 2 proteinDepositorInsertSUMO 2 (SUMO2 Human)
UseTags6XHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-HypaCas9
Plasmid#108294PurposepX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutationsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-eGFP
Plasmid#176861PurposeExpresses cytosolic eGFP in astrocytesDepositorInserteGFP
UseAAVTagsExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gi3-CASE
Plasmid#168122PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A114/E115 within GNAI1PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-NPM WT
Plasmid#17578DepositorInsertNPM (NPM1 Human)
UseTagsEGFP and FlagExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W
Plasmid#67986PurposeCas9 activity reporter with mCherry and BFPDepositorInsertsU6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHA2
Plasmid#85710PurposeThis plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomesDepositorTypeEmpty backboneUseCre/Lox; Herpesvirus cloningTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Snap-Omp25
Plasmid#69599PurposeSnap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondriaDepositorInsertSnap-Omp25
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
LgBiT-ST
Plasmid#207633PurposeA plasmid encoding LgBiT fused to SpyTagDepositorInsertLgBiT-SpyTag
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK V5-LUC Puro (w543-1)
Plasmid#19360Purpose3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, PuroDepositorInsertFirefly Luciferase
UseLentiviral and LuciferaseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pOPH6
Plasmid#40315DepositorInsertPeptide:N-glycosidase F
UseTagsHexaHistidine and OmpA3 leaderExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
PB-gRNA-Puro
Plasmid#121121PurposeExpression of a gRNA together with a Puromycin resistanceDepositorInsertgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)
Plasmid#185911PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
CXCR1-Tango
Plasmid#66259PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertCXCR1 (CXCR1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
spNW50
Plasmid#173486PurposeExpresses spNW50 in Bl21DepositorInsertspNW50
UseTagsHistagExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRL-TetO-Pax7-IRES-EGFP
Plasmid#109372PurposeExpresses Dox-inducible Pax7 together with a GFP reporterDepositorInsertPax7
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
VGAT scFv [L118/14]
Plasmid#206754PurposeMammalian Expression of VGAT scFV. Derived from hybridoma L118/14 scFv.DepositorInsertVGAT (Mus musculus) recombinant scFV (Slc32a1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73037PurposeExpress Nluc and copGFP dual reporterDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CA-E545K
Plasmid#116485PurposeLentiviral expression of PIK3CA E545KDepositorInsertPIK3CA (PIK3CA Human)
UseLentiviralTagsExpressionMutationE545KPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-mScarlet-Gephyrin.FingR-IL2RGTC
Plasmid#125695PurposeGlobal labeling of inhibitory synapses (red fluorescence)DepositorInsertmScarlet-Gephyrin.FingR-IL2RGTC
UseAAVTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+mScarlet-C Cloning Vector
Plasmid#128147PurposeTo express a protein of interest fused to the C-terminus of mScarletDepositorTypeEmpty backboneUseTagsmScarlet fusionExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
FlucWT-HA-GFP11-N1
Plasmid#91954PurposeExpresses FlucWT-HA-GFP11 in mammalian cellsDepositorInsertFlucWT-HA-GFP11
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
mito-EcSTH
Plasmid#211921PurposeMitochondrial localization of EcSTHDepositorInsertmito-EcSTH
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
BPK2660
Plasmid#70709PurposeHuman expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2
Plasmid#174665PurposeExpresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoterDepositorInsertsLUC2
mCherry:T2A:Hygro
UseLentiviral and LuciferaseTagsmCherry and hygromycin dual reporter gene linked …ExpressionMammalianMutationPromoterHuman cytomegalovirus immediate early enhancer/pr…Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET his-eGFP
Plasmid#113551PurposeFor bacterial expression and purification of eGFPDepositorInserthis-eGFP
UseTagsExpressionBacterialMutationA206KPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pABE8e-protein
Plasmid#161788PurposeRhamnose-inducible expression of 8xHIS-ABE8e in E. coliDepositorInsertABE8e base editor
UseTags8xHIS and BPNLSExpressionBacterialMutationPromoterpRhaBADAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A
Plasmid#103865PurposeCatalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).DepositorInsertPspCas13b
UseCRISPR and LentiviralTags3xHA and HIV NESExpressionMammalianMutationContains the H133A and H1058A mutations which ina…PromoterEF1aAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSN067
Plasmid#101748PurposeSingle copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsCpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
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Flag-FOXP3
Plasmid#153147PurposeExpression of Flag tagged human FOXP3 in mammalian cellsDepositorInserthuman FOXP3 (FOXP3 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
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eSpCas9(1.1)-T2A-Puro
Plasmid#101039PurposeEnhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.DepositorTypeEmpty backboneUseCRISPRTags3xFLAG and T2A-PuroExpressionMammalianMutationPromoterCBhAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SV40NLSf-mScarletP2A-hLuc
Plasmid#218793PurposeAAV reporter genome plasmidDepositorInsertNLSf-mScarletP2A-hLuc
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG_PCP-T5Exo
Plasmid#214736Purposeencodes T5 exonuclease with a PP7 coat protein fused to the N terminusDepositorInsertCAG_PCP-T5Exo_bGH
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ADAR2DD_E488Q
Plasmid#205598Purposeexpresses human ADAR2 deaminase domain (E488Q hyperactive mutant) in mammalian cellsDepositorInsertADAR2 deaminase domain (ADARB1 Human)
UseTagsExpressionMammalianMutationE488Q hyperactive mutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RRM2
Plasmid#13796DepositorInsertRRM2 (RRM2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCK302
Plasmid#87768PurposeAs pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be usedDepositorInsertsPrhaBAD-sfGFP
rhaS from E. coli with its native RBS from E. coli
UseSynthetic BiologyTags6xHis TagExpressionBacterialMutationPromoterPrhaBAD rhamnose-inducible promoter from E. coli …Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hygro(+)-mscGAS
Plasmid#102607PurposeMammalian expression of mouse cGASDepositorInsertcGAS (Cgas Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICH47761
Plasmid#48003DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC hTMEM115-3C-3xHA-P2A-eGFP
Plasmid#214996PurposeStably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.DepositorInsertTMEM115 (TMEM115 Human)
UseLentiviralTags3xHAExpressionMammalianMutationPromoterUBCAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-TagRFP
Plasmid#67167PurposeCRISPaint universal donor for TagRFP fusion taggingDepositorInsertTagRFP
UseTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSP2-96-merTetO-EFS-BLaR
Plasmid#118713PurposeContains an optimized 96-mer TetO repeat for imaging of desired lociDepositorInsertsBlasticidin resistance gene
EFS-NS promoter
UseTagsExpressionMammalianMutationPromoterEFS-NSAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP
Plasmid#188777PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-SIRT2-Flag
Plasmid#102623PurposeExpresses full length SIRT2 isoform 1 in mammalian cellsDepositorInsertSirtuin 2 (SIRT2 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WHaloCaMP1a-EGFP
Plasmid#205313PurposeExpression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoterDepositorInsertWHaloCaMP1a-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC G3BP1-SNAP
Plasmid#119949PurposeLentiviral vector expressing G3BP1-SNAP fusion proteinDepositorInsertG3BP1 (G3BP1 Human)
UseLentiviralTagsSNAPExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Halo-KDEL
Plasmid#124316PurposeExpresses ER specific HaloTag proteinDepositorInsertHalo tag
UseTagsKDEL SeqExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LC3
Plasmid#21073PurposeMammalian expression of rat LC3 fused to EGFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagsEGFPExpressionMammalianMutationFirst ATG is not included.PromoterCMVAvailable sinceMay 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAF0432 Ef1a-Dn29-2a-EGFP
Plasmid#193459PurposeExpression of RecombinaseDepositorInsertDn29
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only