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Plasmid#24167PurposeMammalian expression of human JMJD3 with HA tagDepositorInsertJMJD3 (KDM6B Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
Mito-PyronicSF/pCMV-myc-mito
Plasmid#124813PurposeHighly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.DepositorInsertPyronicSF
UseTagsMitochondrial Sequence SignalExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXO1A
Plasmid#153141PurposeExpression of Flag tagged human FOXO1A in mammalian cellsDepositorInserthuman FOXO1 (FOXO1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP
Plasmid#50465PurposeEGFP under the control of human synapsin promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, and AAV9 trial sizeInsertEGFP
UseAAVTagsN/AExpressionMutationPromoterhuman Synapsin 1Available sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLGB30
Plasmid#126620PurposeSuicide vector for allelic replacement in Bacteroides, tetracycline selection and rhamnose-inducible ss-Bfe1 counterselectionDepositorTypeEmpty backboneUseAllellic exchange in bacteriaTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET_RTX-6xHis
Plasmid#145029PurposepET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tagDepositorArticleInsertRTX
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG-Flex-TCB
Plasmid#48332PurposeCre-dependant TVA-mCherry fusionDepositorInsertTVA receptor mCherry
UseAAVTagsmCherry fusion to TVA receptorExpressionMammalianMutationPromoterCAGAvailable sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTTP1B
Plasmid#91723Purposeintegration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNADepositorTypeEmpty backboneUseIntegration-proficient plasmid vectors for mycoba…TagsExpressionMutationPromoterAvailable sinceMay 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-CRISPRoffv2.1-IRES-BlastR
Plasmid#207180PurposeLentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a PromoterDepositorInsertCRISPRoffv2.1
UseCRISPR and LentiviralTagsHA and TagBFPExpressionMammalianMutationPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-D156R
Plasmid#213744PurposeFor circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cellsDepositorInsertLbCas12a
UseCRISPRTagsBPNLSExpressionMammalianMutationD156RPromoterCMVAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-PP7-2xmCherry-CAAX
Plasmid#74925PurposeExpresses PP7-2xmCherry-CAAXDepositorInsertPP7-2xmCherry-CAAX
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dox nontargeting shRNA mKate
Plasmid#155292PurposeLentivirus for doxycycline inducible expression of nontargeting shRNA, mKate marker, based on Addgene 11652DepositorInsertNontargeting shRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ai65(RCFL-tdT) targeting vector
Plasmid#61577PurposeTarget a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locusDepositorInsertpCAG-FSF-LSL-tdTomato
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterCAGAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRDA_888
Plasmid#201165PurposeLentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLSDepositorInsertPuromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB2E
Plasmid#195288PurposeBicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selectionDepositorInsertHSV-TK EGFP 2A Puro
UseTagsExpressionMammalianMutationPromoterEFSAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CreERT2
Plasmid#14797PurposeMammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)DepositorInsertCre-ERT2 fusion protein
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGFPmAID
Plasmid#174213PurposeGFP::mAID donor backboneDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_VP35_EBOV
Plasmid#103050PurposeEncodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertVP35 gene of Ebola virus (VP35 (Zaire ebolavirus, Mayinga))
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-alphasynuclein-A53T
Plasmid#40823DepositorInsertSNCA (SNCA Human)
UseTagsEGFPExpressionMammalianMutationA53T (aggregates more rapidly than the wild-type …PromoterCMVAvailable sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
Anti-GFP [N86/38.1R]
Plasmid#114492PurposeMammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.DepositorInsertanti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Flag-WTAP
Plasmid#53741PurposeFor expression of WTAP (Wilms tumor 1 associated protein) in human cellsDepositorInsertWilms tumor 1 associated protein (WTAP Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterT7Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.LifeAct-Ruby.W
Plasmid#51009PurposeLentivector that expresses LifeAct-Ruby from internal human PGK promoterDepositorInsertLifeAct-mRFPruby
UseLentiviralTagsLifeActExpressionMammalianMutationPromoterhuman PGKAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-PE-bacteria
Plasmid#172715PurposeExpression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editingDepositorInsertCas9n-linker-M-MLV2
UseTagsExpressionBacterialMutationH840A of Cas9PromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKI1
Plasmid#199118PurposeExpresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labellingDepositorInsertH6-SNAP-RapA
UseTags6xHis and SNAP-tagExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TBG-ER-TurboID
Plasmid#149415PurposeHepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorInsertER-TurboID
UseAAVTagsHA and V5ExpressionMutationPromoterTBGAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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MSCV-myc-CAR-2A-Thy1.1
Plasmid#127890PurposeExpresses CART receptor along with tags.DepositorInsertsmyc,
CAR
2A
Thy1.1
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P2
Plasmid#125035PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertPDC1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFG4
Plasmid#87251Purposegene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-HphDepositorInsertHyg
UseTagsparS segmentsExpressionYeastMutationPromoterTEFAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro mouse IRS-1 myc
Plasmid#11374DepositorInsertIRS-1 (Irs1 Mouse)
UseRetroviralTagsmycExpressionMammalianMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only