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Plasmid#215827PurposeExpress CBE6d V106W (with SpCas9) in mammalian cellsDepositorInsertCBE6d V106W-SpCas9-2xUGI (cas9 )
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
TfR-sfGFP-myc tag-SpyCatcher003
Plasmid#133451PurposeExpresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detectionDepositorInsertTfR-sfGFP-myc tag-SpyCatcher003
UseTagsGSSGS and myc tagExpressionMammalianMutationContains C20 and A23 mutations that improve plasm…PromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-WT human MyoD-T2A-dsRedExpress2
Plasmid#60628PurposeExpresses WT human MyoD and dsRedExpress2 in response to doxycyclineDepositorInsertVP64 mouse MyoD (MYOD1 Human)
UseLentiviralTagsExpressionMutationPromoterTREAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcDNA3
Plasmid#18964DepositorInsertFirefly Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-sec61b
Plasmid#186959PurposeExpress sec61b connected with N-terminus HaloTagDepositorInsertSec61 (SEC61B Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-ER GFP
Plasmid#80069PurposeTo express and target GFP into the endoplasmic reticulum using lentivirusDepositorInsertER GFP (SEC61B Human)
UseLentiviralTagsGFP derived from Aequorea coerulescens on the NH2…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_MicroID2-HA
Plasmid#188521PurposeEncodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cellsDepositorInsertMicroID2
UseTransposon mediated gene integrationTagsHAExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
SFFV-HALO-CTERM-GFP
Plasmid#185767PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, HaloExpressionMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(ER-mito)
Plasmid#214423PurposeDetection of ER-mito contact siteDepositorInsertRspAN-FRB-V5-Sec61_IRES_CFAST10-20nm-Halo-mito
UseLentiviralTagsCFAST10-Halo and RspAN-FRB-V5ExpressionMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL1-pCMV7.1
Plasmid#155019PurposeN-terminal 3xFLAG-tagged CUL1 in a pCMV7.1 vectorDepositorInsertCullin 1 (CUL1 Human)
UseTags3xFLAGExpressionBacterialMutationPromoterpCMV7.1Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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MAC_N_IRF1
Plasmid#167760PurposeExpresses IRF1 in mammalian cellsDepositorInsertIRF1 (IRF1 Human)
UseTagsMACExpressionMammalianMutationno mutationsPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro
Plasmid#205631PurposeExpression of TnpBmax in mammalian cellsDepositorInsertISDra2-TnpB optimized for mammalian expression
UseTagsNLS-3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
N8his-GFPenhancer-GGGGS4-LaG16
Plasmid#140442PurposeWe designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.DepositorInsertGFPenhancer-GGGGS4-LaG16
UseTags8 histidine tagExpressionBacterialMutationPromoterT7 promotorAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pet-15b-ultraID
Plasmid#172879Purposeexpresses ultraID in E. coliDepositorInsertultraID
UseTagsmycExpressionBacterialMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP
Plasmid#164038PurposeExpression of dCas9-14xGFP11 in mammalian cellsDepositorInsertdCas9-14xGFP11
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and H840A in Cas9PromoterdSV40Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sox2-t2A-GFP
Plasmid#127537PurposeExpresses human SOX2 and eGFP via t2A linker.DepositorInsertSOX2 (SOX2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)
Plasmid#225756PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -