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Plasmid#29690PurposeGFP reporter protein fused to three mutated GSK3 phosphorylation sitesDepositorInsertGFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
UseTagsFlag and Flag-EGFPExpressionMammalianMutationrecognition sites only, and E3 ligase site, GSK3 …PromoterSP6Available sinceJan. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-BRD4-7D
Plasmid#90007Purposeexpresses 7D mutant BRD4DepositorInsertBRD4 (BRD4 Human)
UseTagsFlagExpressionMammalianMutationChanged 484S to 484D, 488S to 488D, 492S to 492D,…PromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
σ2-mCherry
Plasmid#186579PurposeExpression of adaptor protein 2 (AP2) sigma subunit AP2S1 tagged with mCherryDepositorInsertAP2S1 (AP2S1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPED
Plasmid#162468PurposeDestination Expression vector for dicot plant prime editingDepositorInsertsCas9H840A
attR gateway destination cassette
UseTagsMLV reverse transcriptaseExpressionPlantMutationChange histidine 840 to Alanine based sequence o…Promoter2x35SAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)
Plasmid#174515PurposeRecoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)DepositorInsertsMm PylS
MmtRNA-Pyl-opt(UGA)
UseTagsExpressionBacterialMutationASL from the serT gene (UGA anticodon) and recodedPromoterGlnS and lppAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMF-TetTight
Plasmid#193288PurposeLentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgeneDepositorInsertrtTA-P2A-tTS
UseLentiviralTagsExpressionMammalianMutationPromoterEF‐1αAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF-His-mTET1CD
Plasmid#81053Purposebacterial expression of the catalytic domain of murine TET1DepositorInsertTET1 (Tet1 Mouse)
UseTags6HISExpressionBacterialMutationcatalytic domain amino acid 1367-2057PromoterT7Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-PspCas13b-GEMSgRNA2
Plasmid#204768PurposepU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.DepositorInsertguide RNA targeting GEMS m6A sensor mRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHT-ads
Plasmid#47382PurposeExpresses ads under Pgrac promoter (inducible by IPTG) in bacteriaDepositorInsertads
UseTags6xHisExpressionBacterialMutationPromoterPgracAvailable sinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFR3.v857.SGZ
Plasmid#178330PurposeFluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZDepositorInsertpAAV hSyn iGluSnFR3 v857.SGZ
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and Stargazin and PDZ dom…ExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only