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Plasmid#64108PurposeExpression of Sp dCas9-3XmCherry in mammalian cellsDepositorInsertSp dCas9
UseCRISPR and LentiviralTags3XmCherryExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSicoR
Plasmid#11579DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 EGFP-TEADi
Plasmid#140145PurposeTetracycline-inducible TEADi (GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal)DepositorInsertEGFP TEADi
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA
Plasmid#218528PurposesgRNA targeting human EIF2AK4/GCN2DepositorInsertEIF2AK4 (EIF2AK4 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-CDK11B-V5-miniTurbo_IDG-K
Plasmid#189914PurposeGateway destination clone of CDK11B (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertCDK11B (CDK11B Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUltraHot-mCherry-BLM-WT
Plasmid#206966Purposeexpressing the protein BLM fused to mCherry in human cellsDepositorInsertBloom Syndrome RecQ Like Helicase (BLM Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR.v2.TetON_Cas13d_U6_DR
Plasmid#196727PurposeSortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.DepositorInsertRfxCas13d-T2A-miRFP670nano
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterTREAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro
Plasmid#141367PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp1 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti6-CENP-A-mCherry
Plasmid#89767PurposeLentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-ADepositorInsertCENP-A (CENPA Human)
UseLentiviralTags3xHA and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP
Plasmid#66707Purposeexpresses a single gRNA and VP64-dCas9-VP64DepositorInsertshU6-gRNA expression cassette
S.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64ExpressionMutationD10A and H840APromoterhU6 and hUbCAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE
Plasmid#122151PurposeExpresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.DepositorInsertdCas9 fused to SunTag(24x)
UseLentiviralTags2xNLS-P2A-BFP and NLSExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
ACE2
Plasmid#164219PurposeExpresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection markerDepositorInsertACE2 (ACE2 Human)
UseLentiviralTagsExpressionMutationsilent mutation to remove internal EcoRI site (GA…PromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS4134
Plasmid#131747PurposeHuman reduced folate transporter 1 SLC19A1 for lentiviral transduction, copGFP transduction markerDepositorInsertHuman reduced folate carrier 1 (SLC19A1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.LifeAct-Ruby.W
Plasmid#51009PurposeLentivector that expresses LifeAct-Ruby from internal human PGK promoterDepositorInsertLifeAct-mRFPruby
UseLentiviralTagsLifeActExpressionMammalianMutationPromoterhuman PGKAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF3108
Plasmid#144584PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertASCL2 (ASCL2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
plex.mcs sv40promoter-luc
Plasmid#129429PurposeLuc expression under SV40 promoterDepositorInsertLUC
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PRSX8ChR2 (H134R)eYFP
Plasmid#89539PurposeExpresses ChR2 (H134R) -eYFP in Phox2 (including catecholamine) neuronsDepositorInsertChR2 (H134R)
UseLentiviralTagsEYFPExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcw107-V5
Plasmid#64649Purpose(control) when used to produce lentivirus, express physiological levels of insertDepositorInsertn/a
UseLentiviralTagsV5ExpressionMutationPromoterPGKAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hCaspase-1
Plasmid#208790PurposeInducibly expresses human Caspase1, fused to light-activatable Cry2DepositorInsertCaspase1 (CASP1 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-miniTurbo-V5_pLX304
Plasmid#107176Purposeexpresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vectorDepositorInsertminiTurbo (BirA mutant)
UseLentiviralTagsExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only