Addgene

We narrowed to 11,259 results for: pcDNA

Showing: 2141 - 2160 of 11259 results

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pcDNA3-erExRai-AKAR2

Plasmid

#161756

Purpose

ExRai-AKAR2 targeted to the cytosolic face of the ER membrane.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

CYP450-ExRai-AKAR2

Use

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Pmel-1_TCRB

Plasmid

#175288

Purpose

Expresses Pmel-1 TCR beta chain

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

T cell receptor beta chain (Tcrb Mouse)

Use

Suitable for ivt

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Pmel-1_TCRA

Plasmid

#175287

Purpose

Expresses Pmel-1 TCRA alpha chain

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

T cell receptor alpha chain (Tcra Mouse)

Use

Suitable for ivt

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR-NLS

Plasmid

#64931

Purpose

Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR-NLS (EIF4EBP1 Synthetic, Human)

Use

Tags

Cerulean, Nuclear localization signal (NLS), and …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-Flag-H3.3

Plasmid

#47980

Purpose

tetracycline-inducible expression of human Flag tagged H3F3A

Depositor

Bing Zhu

Article

Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:

Insert

H3F3A (H3-3A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

288 bp T to C synonymous mutation

Promoter

CMV promoter with tetracycline operator

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA6-N-Flag-SREBP1

Plasmid

#134283

Purpose

Expresses Flag-tagged SREBP1 in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SREBP1 (Srebf1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

mouse SREBP1 isoform a precursor

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_FBXO22

Plasmid

#159127

Purpose

expresses FBXO22 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

FBXO22 (FBXO22 Human)

Use

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pZ149-pcDNA3-BhCas12b_v4

Plasmid

#122446

Purpose

Expresses BhCas12b_v4 in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

CMV-NLS-BhCas12b_v4-NLS-3xHA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pri-let-7b

Plasmid

#51378

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

pri-let-7b (MIRLET7B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-Nedd4/2795

Plasmid

#11426

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Nedd4-1 (Nedd4 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

D661N compared to GenBank reference sequence U966…

Promoter

CMV

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-Ataxin3Q28

Plasmid

#22125

Depositor

Henry Paulson

Insert

Ataxin 3 (ATXN3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-YFP-PKD1

Plasmid

#31527

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

Insert

YFP-HA-Protein Kinase D (PRKD1 Human)

Use

Tags

HA and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-H2B-Venus

Plasmid

#87614

Purpose

Venus (YFP) fused to histone H2B.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

H2B-Venus (H2BC8 Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-ICUE3

Plasmid

#61623

Purpose

Plasma membrane-targeted FRET biosensor for monitoring cAMP

Depositor

Jin Zhang

Article

DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

Insert

Lyn-ICUE3 (RAPGEF3 Human)

Use

Tags

ECFP, N-terminal targeting sequence from Lyn kina…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-miniR

Plasmid

#181986

Purpose

MiniR genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniE labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

TfR1-MiniR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) VSFP3.1

Plasmid

#18951

Depositor

Thomas Knopfel

Article

Lundby et al PLoS ONE. 2008 . 3(6):e2514.

Insert

VSFP3.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-eGFP-LIC

Plasmid

#40768

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

Type

Empty backbone

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

Plasmid

#182009

Purpose

CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

IgKchL_HA_STf_myc_PDGFRtmb

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Npl4-Strep-HA

Plasmid

#113495

Purpose

Expression of human Npl4 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

Npl4 (NPLOC4 Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mSTIM1 myc

Plasmid

#17732

Depositor

Anjana Rao

Article

Oh-Hora et al Nat Immunol. 2008 Apr . 9(4):432-43.

Insert

STIM1 (Stim1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only