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Plasmid#227380PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 5YS (TERF1 Human)
UseLentiviralTagsExpressionMutation5YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-3YS-miRFP670-TRF1
Plasmid#227379PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 3YS (TERF1 Human)
UseLentiviralTagsExpressionMutation3YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB50
Plasmid#226311PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with V(T/S)G to AAA CsoS2 MR1-7
UseTagsExpressionBacterialMutationVTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…PromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBWB514
Plasmid#209326Purposep15A-tetR-tet-SapI-ME-RBS-mRFP-ME-U1-Barcode-U2-SapI-dbI terminator-oriT-Cm. Fragment expression vector with Golden Gate sites.DepositorInsertmRFP
UseTagsExpressionBacterialMutationPromotertetAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
TL1 alphaDdCBE A1 (DddA6-C) - TL1 alphaL_DdCBE 2 (left, DddA11-C)
Plasmid#221994PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233A | DddA: T1413IPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd
Plasmid#216170PurposeExpress the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- FOXA.bs- ZEB2.locus
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd
Plasmid#216172PurposeExpress the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- Control- ZEB2.locus
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2
Plasmid#216173PurposeExpress the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- FOXA.bs- ZEB2.locus
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT9050
Plasmid#219429PurposePapE Exonuclease fused to linker 4LF2 as N-terminal tag module (AATG_PapE-4*LF2_AGGT)DepositorInsertAATG_PapE-4xLF2_AGGT
UseMoclo compatible level 0 moduleTagsExpressionMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Gly-tRNA-gRNA scaffold for library 2
Plasmid#192365PurposetRNA sequence provider (for checkpoint library)DepositorInsertGly tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pro-tRNA-gRNA scaffold for library 2
Plasmid#192366PurposetRNA sequence provider (for checkpoint library)DepositorInsertPro tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gly-tRNA-gRNA scaffold for library 1
Plasmid#192368PurposetRNA sequence provider (for immune library)DepositorInsertGly tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pro-tRNA-gRNA scaffold for library 1
Plasmid#192369PurposetRNA sequence provider (for immune library)DepositorInsertPro tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0751
Plasmid#185628PurposeModified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaIDepositorInsertlacZ
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
mt-DdCBE G1333-C
Plasmid#174561Purposemitochondrial-targeting DdCBE (split DddA, G1333-C) Golden Gate destination vectorDepositorInsertDdCBE G1333-C
UseTagsFLAGExpressionPlantMutationPromoterPcUbiAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
mt-DdCBE G1397-C
Plasmid#174563Purposemitochondrial-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vectorDepositorInsertDdCBE G1397-C
UseTagsFLAGExpressionPlantMutationPromoterPcUbiAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
mt-DdCBE G1397-N
Plasmid#174562Purposemitochondrial-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vectorDepositorInsertDdCBE G1397-N
UseTagsHAExpressionPlantMutationPromoterPcUbiAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
mt-DdCBE G1333-N
Plasmid#174560Purposemitochondrial-targeting DdCBE (split DddA, G1333-N) Golden Gate destination vectorDepositorInsertDdCBE G1333-N
UseTagsHAExpressionPlantMutationPromoterPcUbiAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
cp-DdCBE G1397-C
Plasmid#174559PurposeChloroplast-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vectorDepositorInsertDdCBE G1397-C
UseTagsFLAGExpressionPlantMutationPromoterPcUbiAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
cp-DdCBE G1397-N
Plasmid#174558PurposeChloroplast-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vectorDepositorInsertDdCBE G1397-N
UseTagsHAExpressionPlantMutationPromoterPcUbiAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only