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pEPOR0SP0017

Plasmid

#117527

Purpose

Level0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-eCas9 1.0 (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

K810A, K1003A, R1060A

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pJB172

Plasmid

#86992

Purpose

CRISPR/Cas9 in fission yeast using fluoride selection and targetting pil1

Depositor

Julien Berro

Article

Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

Insert

gRNA targeting pil1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF792_2

Plasmid

#86340

Purpose

Encodes gRNA for 3' target of human ZNF792

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF792 (ZNF792 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF652_2

Plasmid

#86317

Purpose

Encodes gRNA for 3' target of human ZNF652

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF652 (ZNF652 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZSCAN9_1

Plasmid

#86312

Purpose

Encodes gRNA for 3' target of human ZSCAN9

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZSCAN9 (ZNF193 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_SP5_1

Plasmid

#86362

Purpose

Encodes gRNA for 3' target of human SP5

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against SP5

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZFP1_iso1_2

Plasmid

#86361

Purpose

Encodes gRNA for 3' target of human ZFP1_iso1

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZFP1_iso1 (ZFP1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZFP1_iso1_1

Plasmid

#86360

Purpose

Encodes gRNA for 3' target of human ZFP1_iso1

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZFP1_iso1 (ZFP1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF792_1

Plasmid

#86339

Purpose

Encodes gRNA for 3' target of human ZNF792

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF792 (ZNF792 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_MIXL1_2

Plasmid

#86336

Purpose

Encodes gRNA for 3' target of human MIXL1

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MIXL1 (MIXL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_MIXL1_1

Plasmid

#86335

Purpose

Encodes gRNA for 3' target of human MIXL1

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MIXL1 (MIXL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF580_2

Plasmid

#86332

Purpose

Encodes gRNA for 3' target of human ZNF580

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF580 (ZNF580 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_ZNF580_1

Plasmid

#86331

Purpose

Encodes gRNA for 3' target of human ZNF580

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF580 (ZNF580 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_ZNF652_1

Plasmid

#86316

Purpose

Encodes gRNA for 3' target of human ZNF652

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF652 (ZNF652 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_ZSCAN9_2

Plasmid

#86313

Purpose

Encodes gRNA for 3' target of human ZSCAN9

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZSCAN9 (ZNF193 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_SAP130_2

Plasmid

#86311

Purpose

Encodes gRNA for 3' target of human SAP130

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against SAP130 (SAP130 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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sgRNA1_pNeurog2-Luciferase reporter

Plasmid

#64160

Purpose

Photoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1 for pNeurog2-Luciferase reporter

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-OR-MalT-3NT

Plasmid

#62309

Purpose

synthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genome

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A2NT sgRNA
Malt-3NT sgRNA
A2NT sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PA2, PPhlF, and pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJK384.4

Plasmid

#155369

Purpose

dCas9 + PA4-mVenus

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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FUCas9GFP

Plasmid

#85555

Purpose

Expresses Cas9 with green fluorescent reporter

Depositor

Marco Herold

Insert

humanised S.pyogenes Cas9

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

hUBC

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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