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Plasmid#117527PurposeLevel0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon)DepositorInsertSp-eCas9 1.0 (no stop codon)
UseCRISPRTagsExpressionMutationK810A, K1003A, R1060APromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJB172
Plasmid#86992PurposeCRISPR/Cas9 in fission yeast using fluoride selection and targetting pil1DepositorInsertgRNA targeting pil1
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF792_2
Plasmid#86340PurposeEncodes gRNA for 3' target of human ZNF792DepositorInsertgRNA against ZNF792 (ZNF792 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF652_2
Plasmid#86317PurposeEncodes gRNA for 3' target of human ZNF652DepositorInsertgRNA against ZNF652 (ZNF652 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZSCAN9_1
Plasmid#86312PurposeEncodes gRNA for 3' target of human ZSCAN9DepositorInsertgRNA against ZSCAN9 (ZNF193 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_SP5_1
Plasmid#86362PurposeEncodes gRNA for 3' target of human SP5DepositorInsertgRNA against SP5
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZFP1_iso1_2
Plasmid#86361PurposeEncodes gRNA for 3' target of human ZFP1_iso1DepositorInsertgRNA against ZFP1_iso1 (ZFP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZFP1_iso1_1
Plasmid#86360PurposeEncodes gRNA for 3' target of human ZFP1_iso1DepositorInsertgRNA against ZFP1_iso1 (ZFP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF792_1
Plasmid#86339PurposeEncodes gRNA for 3' target of human ZNF792DepositorInsertgRNA against ZNF792 (ZNF792 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_MIXL1_2
Plasmid#86336PurposeEncodes gRNA for 3' target of human MIXL1DepositorInsertgRNA against MIXL1 (MIXL1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_MIXL1_1
Plasmid#86335PurposeEncodes gRNA for 3' target of human MIXL1DepositorInsertgRNA against MIXL1 (MIXL1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF580_2
Plasmid#86332PurposeEncodes gRNA for 3' target of human ZNF580DepositorInsertgRNA against ZNF580 (ZNF580 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF580_1
Plasmid#86331PurposeEncodes gRNA for 3' target of human ZNF580DepositorInsertgRNA against ZNF580 (ZNF580 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF652_1
Plasmid#86316PurposeEncodes gRNA for 3' target of human ZNF652DepositorInsertgRNA against ZNF652 (ZNF652 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZSCAN9_2
Plasmid#86313PurposeEncodes gRNA for 3' target of human ZSCAN9DepositorInsertgRNA against ZSCAN9 (ZNF193 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_SAP130_2
Plasmid#86311PurposeEncodes gRNA for 3' target of human SAP130DepositorInsertgRNA against SAP130 (SAP130 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_pNeurog2-Luciferase reporter
Plasmid#64160PurposePhotoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.DepositorInsertsgRNA1 for pNeurog2-Luciferase reporter
UseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-OR-MalT-3NT
Plasmid#62309Purposesynthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genomeDepositorInsertsA2NT sgRNA
Malt-3NT sgRNA
A2NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterPA2, PPhlF, and pBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJK384.4
Plasmid#155369PurposedCas9 + PA4-mVenusDepositorInsertsdCas9 (bacteria)
PA4-mVenus
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
FUCas9GFP
Plasmid#85555PurposeExpresses Cas9 with green fluorescent reporterDepositorInserthumanised S.pyogenes Cas9
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationPromoterhUBCAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only